LOCUS       sequence-279638-        1727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0263
FEATURES             Location/Qualifiers
     source          1..1727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatatggg caacccagga accctttcgc ccaccaaacc tggttcccag
       61 tactatcagt attctagcaa taatccccga aggaggcctc ttcacagtag tgccatggag
      121 gtacagacaa agaaagttcg aaaagttcct ccaggtttgc catcttcagt ctatgctcca
      181 tcagcaagca ctgccgacta caatagggac tcgccaggct atccttcctc caaaccagca
      241 accagcactt tccctagctc cttcttcatg caagatggcc atcacagcag tgacccttgg
      301 agctcctcca gtgggatgaa tcagcctggc tatgcaggaa tgttgggcaa ctcttctcat
      361 attccacagt ccagcagcta ctgtagcctg catccacatg aacgtttgag ctatccatca
      421 cactcctcag cagacatcaa ttccagtctt cctccgatgt ccactttcca tcgtagtggt
      481 acaaaccatt acagcacctc ttcctgtacg cctcctgcca acgggacaga cagtataatg
      541 gcaaatagag gaagcggggc agccggcagc tcccagactg gagatgctct ggggaaagca
      601 cttgcttcga tctattctcc agatcacact aacaacagct tttcatcaaa cccttcaact
      661 cctgttggct ctcctccatc tctctcagca ggcacagctg tttggtctag aaatggagga
      721 caggcctcat cgtctcctaa ttatgaagga cccttacact ctttgcaaag ccgaattgaa
      781 gatcgtttag aaagactgga tgatgctatt catgttctcc ggaaccatgc agtgggccca
      841 tccacagcta tgcctggtgg tcatggggac atgcatggaa tcattggacc ttctcataat
      901 ggagccatgg gtggtctggg ctcagggtat ggaaccggcc ttctttcagc caacagacat
      961 tcactcatgg tggggaccca tcgtgaagat ggcgtggccc tgagaggcag ccattctctt
     1021 ctgccaaacc aggttccggt tccacagctt cctgtccagt ctgcgacttc ccctgacctg
     1081 aacccacccc aggaccctta cagaggcatg ccaccaggac tacaggggca gagtgtctcc
     1141 tctggcagct ctgagatcaa atccgatgac gagggtgatg agaacctgca agacacgaaa
     1201 tcttcggagg acaagaaatt agatgacgac aagaaggata tcaaatcaat tactaggtca
     1261 agatctagca ataatgacga tgaggacctg acaccagagc agaaggcaga gcgtgagaag
     1321 gagcggagga tggccaacaa tgcccgagag cgtctgcggg tccgtgacat caacgaggct
     1381 ttcaaagagc tcggccgcat ggtgcagctc cacctcaaga gtgacaagcc ccagaccaag
     1441 ctcctgatcc tccaccaggc ggtggccgtc atcctcagtc tggagcagca agtccgagaa
     1501 aggaatctga atccgaaagc tgcgtgtctg aaaagaaggg aggaagagaa ggtgtcctca
     1561 gagcctcccc ctctctcctt ggccggccca caccctggaa tgggagacgc atcgaatcac
     1621 atgggacaga tgtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1681 tatatatctt gtggaaagga cgacatgaag agcatatctt gcggacg
//
