LOCUS       sequence-279659-        1658 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1658)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1658)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0279
FEATURES             Location/Qualifiers
     source          1..1658
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcggc cgcgctgagg cccccggctc agggcactgt gacctttgaa
       61 gatgtggctg tgaacttttc ccaggaggaa tggagtctcc ttagtgaggc tcagagatgc
      121 ctttatcatg acgtgatgct ggagaacctg acacttatat cttctctagg ttgttggtat
      181 ggagcaaaag acgagacacc ttctaagcag accctttcta tacaacagga gtccccactc
      241 aggacacatt ggacaggtgt atgtaccaag aaggtccacc tctggggaat gtgtggccct
      301 ctcctgggag atatcttaca ccagggaaca caacacaatc agaaattgaa tgggtttggg
      361 gcatatgaaa aaaaattgga tgacgatgca aaccatcatc aagaccagaa gcagcacatt
      421 ggagagaaat cgtacagaag caatgccaag ggaacatctt ttgtaaagaa ctgtaaattc
      481 catatgtcac atgagccatt tatctttcat gaggttggga aagacttttt gtccagcttg
      541 agattactcc aacaagagga cattcacact tcagggaagt caaactttga aactaagcat
      601 gggatacccc ttcagggtgg aaaaactcat tacatctgtg gagagtccac aataccgttt
      661 agcaacaaac actcacttgt ccttcaccag agacttctcc ctagagaagg accttatgta
      721 tgcagtgatt ctgggaaatt cactagcaaa agtaatagtt ttaataatca tcagggagtt
      781 cgcactggaa aaagacctta tcagtgtgga caatgtgatg aatcattttg gtataaggcc
      841 cacctcactg aacaccagag agttcacact ggagaaagac cttatgagtg tggagaatgt
      901 gataaatctt ttagtcataa gcacagtctt gttgaccatc agcgagttca cactggagaa
      961 agaccttatg aatgtgacga atgtgggaaa tcttttagcc ataagcgcag ccttgttcac
     1021 caccagcgag ttcacactgg agaaagacct tatcagtgtg gagaatgtgg gaaatcgttt
     1081 aatcacaagt gcaacctcat tcagcatcag cgagttcaca ctggagaaag accttttgag
     1141 tgtacggcat gtgggaagtt atttaggagc aactcccacc taaaggaaca ccagagagtt
     1201 cacactggag aaagacccta tgagtgtaaa gaatgtagga aatcatttag gtacaagtca
     1261 cacctcactg aacaccagag agttcacact ggagaaaggc catatgagtg tagagaatgt
     1321 gggaaatgtt ttcatcaaaa gggcagtctc attcaacatc agcagatcca ctctggagaa
     1381 aggccacatg agtgtggaga atgtgggaaa tgttttcatc aaaagggcag tctcattcga
     1441 catcagcaga ttcactctgg agaaaggcca catgagtgtg gagaatgtgg aaagtgtttt
     1501 cgtcaaaagg gaaacctcat taaacatcaa cgagttcaca cgggagaaag acatcatgaa
     1561 tgttagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1621 tgtggaaagg acgagaatct gccaaactga taggaccc
//
