LOCUS       sequence-279662-        1403 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1403)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0283
FEATURES             Location/Qualifiers
     source          1..1403
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggggagaaa aaagattcag attacgagga ttatggatga acgtaacaga
       61 caggtgacat ttacaaagag gaaatttggg ttgatgaaga aggcttatga gctgagcgtg
      121 ctgtgtgact gtgagattgc gctgatcatc ttcaacagca ccaacaagct gttccagtat
      181 gccagcaccg acatggacaa agtgcttctc aagtacacgg agtacaacga gccgcatgag
      241 agccggacaa actcagacat cgtggagacg ttgagaaaga agggccttaa tggctgtgac
      301 agcccagacc ccgatgcgga cgattccgta ggtcacagcc ctgagtctga ggacaagtac
      361 aggaaaatta acgaagatat tgatctaatg atcagcaggc aaagattgtg tgctgttcca
      421 cctcccaact tcgagatgcc agtctccatc ccagtgtcca gccacaacag tttggtgtac
      481 agcaaccctg tcagctcact gggaaacccc aacctattgc cactggctca cccttctctg
      541 cagaggaata gtatgtctcc tggtgtaaca catcgacctc caagtgcagg taacacaggt
      601 ggtctgatgg gtggagacct cacgtctggt gcaggcacca gtgcagggaa cgggtatggc
      661 aatccccgaa actcaccagg tctgctggtc tcacctggta acttgaacaa gaatatgcaa
      721 gcaaaatctc ctcccccaat gaatttagga atgaataacc gtaaaccaga tctccgagtt
      781 cttattccac caggcagcaa gaatacgatg ccatcagtga atcaaaggat aaataactcc
      841 cagtcggctc agtcattggc taccccagtg gtttccgtag caactcctac tttaccagga
      901 caaggaatgg gaggatatcc atcagccatt tcaacaacat atggtaccga gtactctctg
      961 agtagtgcag acctgtcatc tctgtctggg tttaacaccg ccagcgctct tcaccttggt
     1021 tcagtaactg gctggcaaca gcaacaccta cataacatgc caccatctgc cctcagtcag
     1081 ttgggagacc gtaccaccac cccttcgaga tacccacaac acacgcgcca cgaggcgggg
     1141 agatctcctg ttgacagctt gagcagctgt agcagttcgt acgacgggag cgaccgagag
     1201 gatcaccgga acgaattcca ctcccccatt ggactcacca gaccttcgcc ggacgaaagg
     1261 gaaagtccct cagtcaagcg catgcgactt tctgaaggat gggcaacata gtaatgaact
     1321 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgac
     1381 atggagacga tacatgatga atg
//
