LOCUS       sequence-279683-        2381 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1887
FEATURES             Location/Qualifiers
     source          1..2381
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatttatc tgctcttcgc gttgaagaag tacaaaatgt cattaatgct
       61 atgcagaaaa tcttagagtg tcccatctgt ctggagttga tcaaggaacc tgtctccaca
      121 aagtgtgacc acatattttg caaattttgc atgctgaaac ttctcaacca gaagaaaggg
      181 ccttcacagt gtcctttatg taagaatgat ataaccaaaa ggagcctaca agaaagtacg
      241 agatttagtc aacttgttga agagctattg aaaatcattt gtgcttttca gcttgacaca
      301 ggtttggagt atgcaaacag ctataatttt gcaaaaaagg aaaataactc tcctgaacat
      361 ctaaaagatg aagtttctat catccaaagt atgggctaca gaaaccgtgc caaaagactt
      421 ctacagagtg aacccgaaaa tccttccttg caggaaacca gtctcagtgt ccaactctct
      481 aaccttggaa ctgtgagaac tctgaggaca aagcagcgga tacaacctca aaagacgtct
      541 gtctacattg aattgggatc tgattcttct gaagataccg ttaataaggc aacttattgc
      601 agtgtgggag atcaagaatt gttacaaatc acccctcaag gaaccaggga tgaaatcagt
      661 ttggattctg caaaaaaggc tgcttgtgaa ttttctgaga cggatgtaac aaatactgaa
      721 catcatcaac ccagtaataa tgatttgaac accactgaga agcgtgcagc tgagaggcat
      781 ccagaaaagt atcagggtga agcagcatct gggtgtgaga gtgaaacaag cgtctctgaa
      841 gactgctcag ggctatcctc tcagagtgac attttaacca ctcagcagag ggataccatg
      901 caacataacc tgataaagct ccagcaggaa atggctgaac tagaagctgt gttagaacag
      961 catgggagcc agccttctaa cagctaccct tccatcataa gtgactcttc tgcccttgag
     1021 gacctgcgaa atccagaaca aagcacatca gaaaaagtat taacttcaca gaaaagtagt
     1081 gaatacccta taagccagaa tccagaaggc ctttctgctg acaagtttga ggtgtctgca
     1141 gatagttcta ccagtaaaaa taaagaacca ggagtggaaa ggtcatcccc ttctaaatgc
     1201 ccatcattag atgataggtg gtacatgcac agttgctctg ggagtcttca gaatagaaac
     1261 tacccatctc aagaggagct cattaaggtt gttgatgtgg aggagcaaca gctggaagag
     1321 tctgggccac acgatttgac ggaaacatct tacttgccaa ggcaagatct agagggaacc
     1381 ccttacctgg aatctggaat cagcctcttc tctgatgacc ctgaatctga tccttctgaa
     1441 gacagagccc cagagtcagc tcgtgttggc aacataccat cttcaacctc tgcattgaaa
     1501 gttccccaat tgaaagttgc agaatctgcc cagagtccag ctgctgctca tactactgat
     1561 actgctgggt ataatgcaat ggaagaaagt gtgagcaggg agaagccaga attgacagct
     1621 tcaacagaaa gggtcaacaa aagaatgtcc atggtggtgt ctggcctgac cccagaagaa
     1681 tttatgctcg tgtacaagtt tgccagaaaa caccacatca ctttaactaa tctaattact
     1741 gaagagacta ctcatgttgt tatgaaaaca gatgctgagt ttgtgtgtga acggacactg
     1801 aaatattttc taggaattgc gggaggaaaa tgggtagtta gctatttctg ggtgacccag
     1861 tctattaaag aaagaaaaat gctgaatgag catgattttg aagtcagagg agatgtggtc
     1921 aatggaagaa accaccaagg tccaaagcga gcaagagaat cccaggacag aaagatcttc
     1981 agggggctag aaatctgttg ctatgggccc ttcaccaaca tgcccacaga tcaactggaa
     2041 tggatggtac agctgtgtgg tgcttctgtg gtgaaggagc tttcatcatt cacccttggc
     2101 acaggtgtcc acccaattgt ggttgtgcag ccagatgcct ggacagagga caatggcttc
     2161 catgcaattg ggcagatgtg tgaggcacct gtggtgaccc gagagtgggt gttggacagt
     2221 gtagcactct accagtgcca ggagctggac acctacctga taccccagat cccccacagc
     2281 cactactagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2341 tcttgtggaa aggacgaagt cgttagcgag gacatttaga t
//
