LOCUS       sequence-279689-        2132 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1960
FEATURES             Location/Qualifiers
     source          1..2132
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggccaa tgtgggatcg atgtttcaat attggaagcg ctttgattta
       61 cagcagctgc agagagaact cgatgccacc gcaacggtat tggcgaaccg gcaggatgaa
      121 agtgagcagt ccagaaagcg gcttatcgaa cagagccggg agttcaagaa gaacactcca
      181 gaggatttgc gcaagcaggt agcgccgctg ctgaagagtt tccaaggaga gattgatgca
      241 ctgagtaaaa gaagcaagga agctgaagca gctttcttga atgtctacaa aagattgatt
      301 gacgtcccag atcccgtacc agctttggat ctcggacagc aactccagct caaagtgcag
      361 cgcctgcacg atattgaaac agagaaccag aaacttaggg aaactctgga agaatacaac
      421 aaggaatttg ctgaagtgaa aaatcaagag gttacgataa aagcacttaa agagaaaatc
      481 cgagaatatg aacagacact gaagaaccaa gccgaaacca tagctcttga gaaggaacag
      541 aagttacaga atgactttgc agaaaaggag agaaagctgc aggagacaca gatgtccacc
      601 acctcaaagc tggaggaagc tgagcataag gttcagagcc tacaaacagc cctggaaaaa
      661 actcgaacag aattatttga cctgaaaacc aaatacgatg aagaaactac tgcaaaggcc
      721 gacgagattg aaatgatcat gacggacctt gaaagggcaa accagagggc agaggtggct
      781 cagagagagg cggagacctt aagggaacag ctctcatcgg ccaatcactc cctccagctg
      841 gcctcacaga tccagaaggc accagacgtg gccatagagg tgctgacccg ctccagccta
      901 gaagttgagt tggccgccaa ggagcgggag atcgcacagc tggtggagga cgtgcagaga
      961 ctccaggcca gcctcaccaa gctgcgggag aattcggcca gccagatctc acagcttgag
     1021 cagcagctga gcgccaaaaa cagcacactc aaacaactgg aagaaaaact caaaggccag
     1081 gctgactatg aagaggtgaa gaaagagctg aacattctga agtccatgga gtttgcaccg
     1141 tccgagggcg ctgggacaca ggatgcggcc aagcccctgg aggtgctgtt gctggagaag
     1201 aaccgctcgc tgcagtccga gaacgccgcg ctgcgcatct ccaacagcga cctgagcgga
     1261 cgctgtgcgg agctgcaagt ccgtatcact gaggctgtgg ccacagccac tgagcagaga
     1321 gagctgatcg cccgcctgga gcaggacctg agcatcattc agtccatcca gcggcccgat
     1381 gccgagggtg ccgctgagca ccgcctggag aagatcccag agcccatcaa agaggccact
     1441 gccctattct acggacctgc agcaccagcc agcggtgccc tcccagaggg ccaggtggat
     1501 tcactgcttt ccatcatctc cagccagagg gagcgcttcc gtgcccggaa ccaggagctt
     1561 gaggccgaga accgcctggc ccagcacacc ctccaggccc tgcagagtga gctggacagc
     1621 ctgcgcgccg acaacatcaa gctctttgag aagatcaagt tcctgcagag ctaccctggc
     1681 cggggcagcg gcagtgatga cacggagctg cggtactcgt cccagtacga ggagcgcctg
     1741 gaccccttct cctccttcag caagcgggag cggcagagga agtacctgag cttgagtccc
     1801 tgggacaagg ccaccctcag catggggcgt ctggttctct ccaacaagat ggcgcgcacc
     1861 atcggcttct tctacacact gttcctgcac tgcctggtct tcctggtgct ctacaagctg
     1921 gcatggagcg agagcatgga gagggactgt gccaccttct gcgccaagaa gttcgctgac
     1981 cacctgcaca agttccacga gaatgacaac ggggctgcgg ctggtgactt gtggcagtag
     2041 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     2101 aaggacgata ggattagaat taaggctcgg ac
//
