LOCUS       sequence-279692-        2000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1964
FEATURES             Location/Qualifiers
     source          1..2000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggccaa tgtgggatcg atgtttcaat attggaagcg ctttgattta
       61 cagcagctgc agagagaact cgatgccacc gcaacggtat tggcgaaccg gcaggatgaa
      121 agtgagcagt ccagaaagcg gcttatcgaa cagagccggg agttcaagaa gaacactcca
      181 gaggatttgc gcaagcaggt agcgccgctg ctgaagagtt tccaaggaga gattgatgca
      241 ctgagtaaaa gaagcaagga agctgaagca gctttcttga atgtctacaa aagattgatt
      301 gacgtcccag aggttacgat aaaagcactt aaagagaaaa tccgagaata tgaacagaca
      361 ctgaagaacc aagccgaaac catagctctt gagaaggaac agaagttaca gaatgacttt
      421 gcagaaaagg agagaaagct gcaggagaca cagatgtcca ccacctcaaa gctggaggaa
      481 gctgagcata aggttcagag cctacaaaca gccctggaaa aaactcgaac agaattattt
      541 gacctgaaaa ccaaatacga tgaagaaact actgcaaagg ccgacgagat tgaaatgatc
      601 atgacggacc ttgaaagggc aaaccagagg gcagaggtgg ctcagagaga ggcggagacc
      661 ttaagggaac agctctcatc ggccaatcac tccctccagc tggcctcaca gatccagaag
      721 gcaccagacg tggagcaggc catagaggtg ctgacccgct ccagcctaga agttgagttg
      781 gccgccaagg agcgggagat cgcacagctg gtggaggacg tgcagagact ccaggccagc
      841 ctcaccaagc tgcgggagaa ttcggccagc cagatctcac agcttgagca gcagctgagc
      901 gccaaaaaca gcacactcaa acaactggaa gaaaaactca aaggccaggc tgactatgaa
      961 gaggtgaaga aagagctgaa cattctgaag tccatggagt ttgcaccgtc cgagggcgct
     1021 gggacacagg atgcggccaa gcccctggag gtgctgttgc tggagaagaa ccgctcgctg
     1081 cagtccgaga acgccgcgct gcgcatctcc aacagcgacc tgagcggacg ctgtgcggag
     1141 ctgcaagtcc gtatcactga ggctgtggcc acagccactg agcagagaga gctgatcgcc
     1201 cgcctggagc aggacctgag catcattcag tccatccagc ggcccgatgc cgagggtgcc
     1261 gctgagcacc gcctggagaa gatcccagag cccatcaaag aggccactgc cctattctac
     1321 ggacctgcag caccagccag cggtgccctc ccagagggcc aggtggattc actgctttcc
     1381 atcatctcca gccagaggga gcgcttccgt gcccggaacc aggagcttga ggccgagaac
     1441 cgcctggccc agcacaccct ccaggccctg cagagtgagc tggacagcct gcgcgccgac
     1501 aacatcaagc tctttgagaa gatcaagttc ctgcagagct accctggccg gggcagcggc
     1561 agtgatgaca cggagctgcg gtactcgtcc cagtacgagg agcgcctgga ccccttctcc
     1621 tccttcagca agcgggagcg gcagaggaag tacctgagct tgagtccctg ggacaaggcc
     1681 accctcagca tggggcgtct ggttctctcc aacaagatgg cgcgcaccat cggcttcttc
     1741 tacacactgt tcctgcactg cctggtcttc ctggtgctct acaagctggc atggagcgag
     1801 agcatggaga gggactgtgc caccttctgc gccaagaagt tcgctgacca cctgcacaag
     1861 ttccacgaga atgacaacgg ggctgcggct ggtgacttgt ggcagtagta atgaactagt
     1921 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaac
     1981 agacctgtaa tagagtccct
//
