LOCUS       sequence-279695-        2333 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1973
FEATURES             Location/Qualifiers
     source          1..2333
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1994..2003
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgagcctctc catgagagat ccggtcattc ctgggacaag catggcctac
       61 catccgttcc tacctcaccg ggcgccggac ttcgccatga gcgcggtgct gggtcaccag
      121 ccgccgttct tccccgcgct gacgctgcct cccaacggcg cggcggcgct ctcgctgccg
      181 ggcgccctgg ccaagccgat catggatcaa ttggtggggg cggccgagac cggcatcccg
      241 ttctcctccc tggggcccca ggcgcatctg aggcctttga agaccatgga gcccgaagaa
      301 gaggtggagg acgaccccaa ggtgcacctg gaggctaaag aactttggga tcagtttcac
      361 aagcggggca ccgagatggt cattaccaag tcgggaaggc gaatgtttcc tccatttaaa
      421 gtgagatgtt ctgggctgga taaaaaagcc aaatacattt tattgatgga cattatagct
      481 gctgatgact gtcgttataa atttcacaat tctcggtgga tggtggctgg taaggccgac
      541 cccgaaatgc caaagaggat gtacattcac ccggacagcc ccgctactgg ggaacagtgg
      601 atgtccaaag tcgtcacttt ccacaaactg aaactcacca acaacatttc agacaaacat
      661 ggatttactt tggccttccc aagtgatcac gctacgtggc aggggaatta tagttttggt
      721 actcagacta tattgaactc catgcacaaa taccagcccc ggttccacat tgtaagagcc
      781 aatgacatct tgaaactccc ttatagtaca tttcggacat acttgttccc cgaaactgaa
      841 ttcatcgctg tgactgcata ccagaatgat aagataaccc agttaaaaat agacaacaac
      901 ccttttgcaa aaggtttccg ggacactgga aatggccgaa gagaaaaaag aaaacagctc
      961 accctgcagt ccatgagggt gtttgatgaa agacacaaaa aggagaatgg gacctctgat
     1021 gagtcctcca gtgaacaagc agctttcaac tgcttcgccc aggcttcttc tccagccgcc
     1081 tccactgtag ggacatcgaa cctcaaagat ttatgtccca gcgagggtga gagcgacgcc
     1141 gaggccgaga gcaaagagga gcatggcccc gaggcctgcg acgcggccaa gatctccacc
     1201 accacgtcgg aggagccctg ccgtgacaag ggcagccccg cggtcaaggc tcaccttttc
     1261 gctgctgagc ggccccggga cagcgggcgg ctggacaaag cgtcgcccga ctcacgccat
     1321 agccccgcca ccatctcgtc cagcactcgc ggcctgggcg cggaggagcg caggagcccg
     1381 gttcgcgagg gcacagcgcc ggccaaggtg gaagaggcgc gcgcgctccc gggcaaggag
     1441 gccttcgcgc cgctcacggt gcagacggac gcggccgccg cgcacctggc ccagggcccc
     1501 ctgcctggcc tcggcttcgc cccgggcctg gcgggccaac agttcttcaa cgggcacccg
     1561 ctcttcctgc accccagcca gtttgccatg gggggcgcct tctccagcat ggcggccgct
     1621 ggcatgggtc ccctcctggc cacggtttct ggggcctcca ccggtgtctc gggcctggat
     1681 tccacggcca tggcctctgc cgctgcggcg cagggactgt ccggggcgtc cgcggccacc
     1741 ctgcccttcc acctccagca gcacgtcctg gcctctcagg gcctggccat gtcccctttc
     1801 ggaagcctgt tcccttaccc ctacacgtac atggccgcag cggcggccgc ctcctctgcg
     1861 gcagcctcca gctcggtgca ccgccacccc ttcctcaatc tgaacaccat gcgcccgcgg
     1921 ctgcgctaca gcccctactc catcccggtg ccggtcccgg acggcagcag tctgctcacc
     1981 accgccctgc cctccatggc ggcggccgcg gggcccctgg acggcaaagt cgccgccctg
     2041 gccgccagcc cggcctcggt ggcagtggac tcgggctctg aactcaacag ccgctcctcc
     2101 acgctctcct ccagctccat gtccttgtcg cccaaactct gcgcggagaa agaggcggcc
     2161 accagcgaac tgcagagcat ccagcggttg gttagcggct tggaagccaa gccggacagg
     2221 tcccgcagcg cgtccccgta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     2281 tggctttata tatcttgtgg aaaggacgaa cgtatctggg tgtgttccag tct
//
