LOCUS       sequence-279715-       12997 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12997)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0006
FEATURES             Location/Qualifiers
     source          1..12997
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    8854..9442
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8907..8927)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9325..9336)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9514..9747
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9825..9946
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9862..9881)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9916..9935
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9986..10121
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10141..10160)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10142..10160)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10170..10187)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10170..10186)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10179..10201)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10328..10783
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10415..10434)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10625..10646
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10809..10913
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10914..10982,10983..11774)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11132..11151)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11945..12533
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12434..12453
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12687..12704
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12821..12842
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12857..12862,12863..12880,12881..12887)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12895..12911
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12900..12922
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12919..12935
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12919..12935
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12954..12974
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12956..12974
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgtat gcagcagtgg aacatgggcc
     4141 tgtgctttgc agcgattcca acatcctgtg cctgtcctgg aaggggcgtg tccccaagag
     4201 tgagaaggag aagcctgtgt gcaggagacg ctactatgag gaaggctggc tggccacggg
     4261 caacgggcga ggagtggttg gggtgacttt cacctctagt cactgtcgca gggacaggag
     4321 tactccacag aggataaatt tcaacctccg gggccacaat agcgaggttg tgctggtgag
     4381 gtggaatgag ccctaccaga aactggccac gtgcgatgcg gacggaggca tattcgtgtg
     4441 gattcagtac gagggcaggt ggtctgtgga gctggtcaac gaccgcgggg cgcaggtgag
     4501 tgatttcacg tggagccatg atggaactca agcacttatt tcctatcgag atgggtttgt
     4561 cctggttggg tctgtcagtg gacaaagaca ctggtcatcc gaaatcaact tggaaagtca
     4621 aattacgtgt ggcatatgga ctcctgacga ccaacaggtg ctgtttggca cggccgatgg
     4681 gcaggtgatt gtcatggatt gccacggcag aatgctggcc cacgtcctct tgcacgagtc
     4741 agacggtgtc ctcggcatgt cctggaacta cccgatcttc ctggtggagg acagcagcga
     4801 gagcgacacg gactcagatg actacgcccc tccccaagat ggtccggcag catatcccat
     4861 cccagtgcag aacatcaagc ctctgctcac cgtcagcttc acctcgggag acatcagctt
     4921 aatgaacaac tacgatgact tgtctcccac ggtcatccgc tcagggctga aagaggtggt
     4981 agcccagtgg tgcacacagg gggacttgct ggcagtcgct gggatggaac ggcagaccca
     5041 gcttggtgag cttcccaatg gtccccttct gaagagtgcc atggtcaagt tctacaatgt
     5101 tcgtggggag cacatcttca cactggacac tctcgtgcag cgccccatca tctccatctg
     5161 ctggggtcac cgggattcga ggctgttgat ggcatcagga ccagccctgt acgtggtgcg
     5221 tgtggagcac cgggtgtcca gcctgcagct gctgtgccag caggccatcg ccagcacctt
     5281 gcgtgaggac aaggacgtca gcaagctgac tctgcccccc cgcctctgct cctacctctc
     5341 cactgccttc atccccacca tcaagccccc aattccagat ccgaacaaca tgagagactt
     5401 tgtcagctac ccatcagccg gcaacgagcg gctgcactgc accatgaagc gcacagagga
     5461 cgacccggag gtgggcggcc cgtgctacac gctctacctg gagtacctgg gcgggcttgt
     5521 gcccatcctc aaagggcggc gcatcagcaa gctgcggcca gagttcgtca tcatggaccc
     5581 gcggacagat agcaaaccag atgaaatcta tgggaacagc ttgatttcta ctgtgatcga
     5641 cagctgcaac tgctcagact ccagtgacat tgagctgagt gatgactggg ctgccaagaa
     5701 atctcccaaa atctccagag ctagcaaatc acccaaactc ccaaggatca gcattgaggc
     5761 ccgcaagtca cccaagctgc cccgggctgc tcaggagctc tcccggtccc cacggttgcc
     5821 cctgcgcaag ccctctgtgg gctcgcccag cctgactcgg agagagtttc cttttgaaga
     5881 catcactcag cacaactatc ttgctcaggt cacgtctaat atctggggaa ccaaatttaa
     5941 gattgtgggc ttggctgctt tcctgccaac caacctcggt gcagtaatct ataaaaccag
     6001 cctcctgcat ctccagccgc ggcagatgac catttatctc ccagaagttc ggaaaatttc
     6061 catggactat attaatttac ctgtcttcaa cccaaatgtt ttcagtgaag atgaagatga
     6121 tttaccagtg acaggagcat ctggtgtccc tgagaacagc ccaccttgta ccgtgaacat
     6181 ccctattgca ccgatccaca gctcggctca ggctatgtcc cccacgcaga gcatagggct
     6241 ggtgcagtcc ctactggcca atcagaatgt gcagctagat gtcctgacca accagacgac
     6301 agctgtaggg acagcagaac atgcaggtga cagtgccacc cagtacccag tctccaaccg
     6361 gtactccaat cctggacagg tgattttcgg aagcgtggaa atgggccgca tcattcagaa
     6421 cccccctcca ctgtccctgc ctcccccgcc gcaggggccc atgcagctgt ccacggtggg
     6481 ccatggagac cgagaccacg aacacctgca gaagtcagcc aaggccctgc ggccaacacc
     6541 gcagctggca gctgaggggg acgcagtggt ctttagtgcc ccccaggagg tccaggtgac
     6601 gaagataaac cctccacccc cgtacccagg aaccatcccc gctgccccca ccacagcagc
     6661 acccccgccc cctctgccgc ccccacagcc cccagtggat gtgtgcttga agaagggcga
     6721 cttctccctc taccccacgt cagtgcacta ccagaccccc ctgggctatg agaggatcac
     6781 caccttcgac agcagtggca acgtggagga ggtgtgccgg ccccgcaccc ggatgctgtg
     6841 ctcccagaac acgtacaccc tccccggccc gggtagctct gccaccttga ggctcacggc
     6901 cactgagaag aaggtccctc agccctgcag cagtgccacc ctgaaccgcc tgaccgtccc
     6961 tcgctactcc atccccaccg gggacccacc cccgtatcct gaaattgcca gccagctggc
     7021 ccaggggcgg ggggctgccc agaggtccga caatagcctc atccacgcta ccctgcggag
     7081 gaacaaccgt gaggctacgc tcaagatggc ccagctggcc gacagcccgc gggcccccct
     7141 gcagcccctg gccaagtcca agggcgggcc cgggggggtg gtgacacagc tcccagcgcg
     7201 gcccccacct gccctgtaca cctgcagtca gtgcagtggc acagggccca gctcacagcc
     7261 cggagcctcc ctggcccata ccgccagcgc ctccccgttg gcctcccagt cctcctacag
     7321 cctcctgagc ccacccgaca gcgcccgcga ccgcaccgac tacgtcaact cggccttcac
     7381 ggaggacgag gccctgtccc agcactgtca gcttgagaag cccttgaggc accctcccct
     7441 gcctgaagct gctgtcaccc tgaaacggcc acccccttac cagtgggacc ccatgctggg
     7501 tgaggatgtt tgggttcctc aagaaaggac agcacagact tcagggccca accccttaaa
     7561 actgtcctct ctgatgctga gtcagggcca gcacctggac gtgtcccgac tgcccttcat
     7621 ctcccccaag tctcctgcca gccccactgc cactttccaa acaggctatg ggatgggagt
     7681 gccatatcca ggaagctata acaacccccc tttgcctgga gtgcaggctc cctgctctcc
     7741 caaagatgcc ctgtccccaa cgcagtttgc acaacaggag cctgctgtgg tccttcagcc
     7801 gctgtaccca cccagcctct cctattgcac cctgcccccc atgtacccag gaagcagcac
     7861 gtgctctagt ttacagctgc cacctgtcgc cttgcatcca tggagttcct acagcgcctg
     7921 cccgcccatg cagaaccccc agggcactct ccccccaaag ccacacttgg tggtggagaa
     7981 gccccttgtg tccccaccac ctgccgacct ccaaagccac ttgggcacag aggtgatggt
     8041 agagactgca gacaacttcc aggaagtcct ctccctgacc gaaagcccag tcccccagcg
     8101 gacagaaaaa tttggaaaga agaaccggaa gcgcctggac agccgagcag aagaaggcag
     8161 cgttcaggcc atcactgagg gcaaagtgaa gaaggaggct aggactttga gtgactttaa
     8221 ttccctaatc tccagcccac acctggggag agagaagaag aaagtgaaga gtcagaaaga
     8281 ccaactgaag tcaaagaagt tgaataagac aaacgagttc caggacagct ccgagagcga
     8341 gcctgagctg ttcatcagcg gggatgagct catgaaccag agccagggca gcagaaaggg
     8401 ctggaaaagc aagcgctccc cacgggccgc cggcgagctg gaggaggcca agtgccggcg
     8461 ggccagtgag aaggaggacg ggcggctggg cagccaaggc ttcgtgtacg tgatggccaa
     8521 caagcagccg ctgtggaacg aggccaccca ggtctaccag ctggacttcg gggggcgggt
     8581 gacccaggag tccgccaaga acttccagat tgagttagag gggcggcagg tgatgcagtt
     8641 tggacggatt gatggcagtg cgtacattct agacttccag tatccgttct cagccgtgca
     8701 ggcctttgca gttgccctgg ccaacgtgac tcagcgcctc aaatagtaat gaactagtcc
     8761 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgataacac
     8821 agtgcggcgc tcccgcgtac gcgttaagtc gacaatcaac ctctggatta caaaatttgt
     8881 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     8941 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     9001 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
     9061 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
     9121 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
     9181 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
     9241 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
     9301 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
     9361 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
     9421 tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag gcagctgtag
     9481 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     9541 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     9601 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     9661 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     9721 tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat cttattattc
     9781 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
     9841 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     9901 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     9961 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
    10021 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
    10081 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
    10141 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
    10201 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
    10261 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
    10321 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
    10381 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
    10441 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
    10501 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
    10561 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
    10621 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
    10681 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
    10741 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
    10801 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
    10861 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
    10921 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
    10981 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
    11041 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
    11101 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
    11161 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
    11221 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
    11281 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
    11341 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
    11401 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
    11461 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
    11521 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
    11581 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
    11641 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
    11701 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
    11761 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
    11821 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
    11881 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
    11941 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
    12001 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
    12061 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
    12121 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
    12181 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
    12241 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
    12301 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
    12361 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
    12421 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
    12481 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
    12541 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
    12601 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
    12661 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
    12721 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
    12781 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
    12841 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
    12901 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
    12961 accctcacta aagggaacaa aagctggagc tgcaagc
//
