LOCUS       sequence-27973-e         686 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Cheriyath V, Roy AL
  TITLE     Alternatively spliced isoforms of TFII-I. Complex formation, nuclear
            translocation, and differential gene regulation.
  JOURNAL   J Biol Chem. 2000 Aug 25. 275(34):26300-8.
  PUBMED    10854432
REFERENCE   2  (bases 1 to 686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Aug 25. 275(34):26300-8."
COMMENT     Sequence Label: pEBG-TFII-I alpha
FEATURES             Location/Qualifiers
     source          1..686
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..39
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..66
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ggatccacca tgggcagcag ccatcatcat catcatcaca gcagcggcct ggtgccgcgc
       61 ggcagccata tggcccaagt tgcaatgtcc accctccccg ttgaagatga ggagtcctcg
      121 gagagcagga tggtggtgac attcctcatg tcagctctcg agtccatgtg taaagaactg
      181 gccaagtcca aagccgaagt ggcctgcatt gcagtgtatg aaacagacgt gtttgtcgtc
      241 ggaactgaaa gaggacgtgc ttttgtcaat accagaaagg attttcaaaa agattttgta
      301 aaatattgtg ttgaagaaga agaaaaagct gcagagatgc ataaaatgaa atctacaacc
      361 caggcaaatc ggatgagtgt agatgctgta gaaattgaaa cactcagaaa aacagttgag
      421 gactatttct gcttttgcta tgggaaagct ttaggcaaat ccacagtggt acctgtacca
      481 tatgagaaga tgctgcgaga ccagtcggct gtggtagtgc aggggcttcc ggaaggtgtt
      541 gcctttaaac accccgagaa ctatgatctt gcaaccctgn nnnnngattt tgganaacaa
      601 annnngnatt tcattcatca ttaagaganc ttttttagag ccaaagaagc atgtaggtgg
      661 tcgtgtgatg gtaacagatg ctgaca
//
