LOCUS       sequence-279737-        3275 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0092
FEATURES             Location/Qualifiers
     source          1..3275
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggatgg ccccaggtgt aagcgcagaa agcaggcgaa cccgcggcgc
       61 aataacggaa aggaagggca agaaatcctg gggcctgaag ctcaggcaga tgaagcagga
      121 tgtacagtaa aagatgatga atgcgagtca gatgcagaaa atgagcaaaa ccatgatcct
      181 aatgttgaag agtttctaca acaacaagac actgctgtca tttttcctga ggcacctgaa
      241 gaggaccaga ggcagggcac accagaagcc agtggtcatg atgaaaatgg aacaccagat
      301 gcattttcac aattactcac ctgtccatat tgtgatagag gctataaacg ctttacctct
      361 ctgaaagaac acattaaata tcgtcatgaa aagaatgaag ataactttag ttgctccctg
      421 tgcagttaca cctttgcata cagaacccaa cttgaacgtc acatgacatc acataaatca
      481 ggaagagatc aaagacatgt gacgcagtct gggtgtaatc gtaaattcaa atgcactgag
      541 tgtggaaaag ctttcaaata caaacatcac ctaaaagagc acttaagaat tcacagtgga
      601 gagaagccat atgaatgccc aaactgcaag aaacgctttt cccattctgg ctcctatagc
      661 tcacacataa gcagtaagaa atgtatcagc ttgatacctg tgaatgggcg accaagaaca
      721 ggactcaaga catctcagtg ttcttcaccg tctctttcag catcaccagg cagtcccaca
      781 cgaccacaga tacggcaaaa gatagagaat aaaccccttc aagaacaact ttctgttaac
      841 caaattaaaa ctgaacctgt ggattatgaa ttcaaaccca tagtggttgc ttcaggaatc
      901 aactgttcaa cccctttaca aaatggggtt ttcactggtg gtggcccatt acaggcaacc
      961 agttctcctc agggcatggt gcaagctgtt gttctgccaa cagttggttt ggtgtctccc
     1021 ataagtatca atttaagtga tattcagaat gtacttaaag tggcggtaga tggtaatgta
     1081 ataaggcaag tgttggagaa taatcaagcc aatcttgcat ccaaagaaca agaaacaatc
     1141 aatgcttcac ccatacaaca aggtggccat tctgttattt cagccatcag tcttcctttg
     1201 gttgatcaag atggaacaac caaaattatc atcaactaca gtcttgagca gcctagccaa
     1261 cttcaagttg ttcctcaaaa tttaaaaaaa gaaaatccag tcgctacaaa cagttgtaaa
     1321 agtgaaaagt taccagaaga tcttactgtt aagtctgaga aggacaaaag ctttgaaggg
     1381 ggggtgaatg atagcacttg tcttctgtgt gatgattgtc caggagatat taatgcactt
     1441 ccagaattaa agcactatga cctaaagcag cctactcagc ctcctccact ccctgcagca
     1501 gaagctgaga agcctgagtc ctctgtttca tcagctactg gagatggcaa tttgtctcct
     1561 agtcagccac ctttaaagaa cctcttgtct ctcctaaaag catattatgc tttgaatgca
     1621 caaccaagtg cagaagagct ctcaaaaatt gctgattcag taaacctacc actggatgta
     1681 gtaaaaaagt ggtttgaaaa gatgcaagct ggacagattt cagtgcagtc ttctgaacca
     1741 tcttctcctg aaccaggcaa agtaaatatc cctgccaaga acaatgatca gcctcaatct
     1801 gcaaatgcaa atgaacccca ggacagcaca gtaaatctac aaagtccttt gaagatgact
     1861 aactccccag ttttaccagt gggatcaacc accaatggtt ccagaagtag tacaccatcc
     1921 ccatcacctc taaacctttc ctcatccaga aatacacagg gttacttgta cacagctgag
     1981 ggtgcacaag aagagccaca agtagaacct cttgatcttt cactaccaaa gcaacaggga
     2041 gaattattag aaaggtcaac tatcactagt gtttaccaga acagtgttta ttctgtccag
     2101 gaagaaccct tgaacttgtc ttgcgcaaaa aaggagccac aaaaggacag ttgtgttaca
     2161 gactcagaac cagttgtaaa tgtaatccca ccaagtgcca accccataaa tatcgctata
     2221 cctacagtca ctgcccagtt acccacaatc gtggccattg ctgaccagaa cagtgttcca
     2281 tgcttaagag cgctagctgc caataagcaa acgattctga ttccccaggt ggcatacacc
     2341 tactcaacta cggtcagccc tgcagtccaa gaaccaccct tgaaagtgat ccagccaaat
     2401 ggaaatcagg atgaaagaca agatactagc tcagaaggag tatcaaatgt agaggatcag
     2461 aatgactctg attctacacc gcccaaaaag aaaatgcgga agacagaaaa tggaatgtat
     2521 gcttgtgatt tgtgtgacaa gatattccaa aagagtagtt cattattgag acataaatat
     2581 gaacacacag gtaaaagacc tcatgagtgt ggaatctgta aaaaggcatt taaacacaaa
     2641 catcatttga ttgaacacat gcgattacat tctggagaaa agccctatca atgtgacaaa
     2701 tgtggaaagc gcttctcaca ctctgggtct tattctcaac acatgaatca tcgctactcc
     2761 tactgtaaga gagaagcgga agaacgtgac agcacagagc aggaagaggc agggcctgaa
     2821 atcctctcga atgagcacgt gggtgccagg gcgtctccct cacagggcga ctcggacgag
     2881 agagagagtt tgacaaggga agaggatgaa gacagtgaaa aagaggaaga ggaggaggat
     2941 aaagagatgg aagaattgca ggaagaaaaa gaatgtgaaa aaccacaagg ggatgaggaa
     3001 gaggaggagg aggaggaaga agtggaagaa gaagaggtag aagaggcaga gaatgaggga
     3061 gaagaagcaa aaactgaagg tctgatgaag gatgacaggg ctgaaagtca agcaagcagc
     3121 ttaggacaaa aagtaggcga gagtagtgag caagtgtctg aagaaaagac aaatgaagcc
     3181 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     3241 ggaaaggacg actacctgta agtgaggcca cgtgc
//
