LOCUS       sequence-279758-        1556 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0129
FEATURES             Location/Qualifiers
     source          1..1556
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccacgtc tatactcggg gaagagccgc gcttcggaac gaccccgttg
       61 gccatgctgg cggcgacctg caacaagatc ggcaacacga gcccgctgac gacgctgcca
      121 gagtcgagcg ccttcgccaa aggcggcttt cacccctgga agcgctcctc gtccagctgc
      181 aacctcggct ccagcctctc gggcttcgcg gtggccaccg ggggccgtgg ctcgggcggc
      241 ctggcgggcg gctcgggcgc cgccaacagc gccttctgcc tggcctccac gtcgcccacg
      301 tcgtccgcct tcagcagcga ctacggcggc ctcttctcca actcggcggc tgccgcggcg
      361 gcagcggccg gggtgtcccc gcaggaggcg ggtggccagt cggccttcat ttccaaggtg
      421 cacacgacgg cagccgacgg gctgtacccg cgcgtgggca tggcgcaccc gtacgagtcc
      481 tggtacaagt cgggcttcca ttcgacgctg gcggccggcg aggtgaccaa cggcgcggcg
      541 tcgtcgtggt gggacgtgca cagcagcccg ggctcgtggc tggaagtgca gaaccccgct
      601 ggggggctcc agagctcgct gcactcgggc gccccccagg cctcgctgca ctcgcagctg
      661 ggcacctaca accccgactt cagctcgctc acgcactccg ccttcagctc cacgggcctc
      721 ggctcctccg ccgccgccgc ctcccacctg ctctccacca gccagcacct gctggcccag
      781 gacggcttca agccggtgtt gccctcctat tcggactcca gcgccgccgt ggcagccgcc
      841 gccgccagcg ccatgatatc gggcgccgcg gctgccgccg ccggggggag ctcggcacgc
      901 tctgcccgcc gctactcggg ccgcgccacc tgcgactgcc ccaactgcca ggaggcggag
      961 cggctgggcc cggccggggc gagcctgcgg cgcaagggcc tgcacagctg ccacattccg
     1021 ggctgcggca aggtgtacgg gaagacgtcg cacctgaagg cgcacctgcg ctggcacacg
     1081 ggcgagcggc ccttcgtgtg caactggctc ttctgcggca agcgcttcac gcgctcggac
     1141 gagctgcagc ggcatctgcg gactcacacg ggcgagaagc gcttcgcctg tccggtgtgc
     1201 aacaagcgct tcatgcgcag cgaccacctg agcaaacaca ttaagacgca caacgggggc
     1261 ggcgggggca aaaagggcag cgacagtgac acggacgcca gcaacctgga gacgccccgt
     1321 tccgaatccc ccgacctcat cctgcatgac tccggcgtca gtgccgcccg ggcggcggca
     1381 gcggcggcgg cggcagcggc ggcggcggcg gcggcggcct ccgcgggagg caaggaagca
     1441 gcgtctggcc ccaacgactc ttagtaatga actagtccgt aacttgaaag tatttcgatt
     1501 tcttggcttt atatatcttg tggaaaggac gaaaggagcg cggatatgat cgcaca
//
