LOCUS       sequence-27976-e         712 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Cheriyath V, Roy AL
  TITLE     Alternatively spliced isoforms of TFII-I. Complex formation, nuclear
            translocation, and differential gene regulation.
  JOURNAL   J Biol Chem. 2000 Aug 25. 275(34):26300-8.
  PUBMED    10854432
REFERENCE   2  (bases 1 to 712)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Aug 25. 275(34):26300-8."
COMMENT     Sequence Label: pEBG-TFII-I beta
FEATURES             Location/Qualifiers
     source          1..712
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctggttccgc gtgganccac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatggcccaa gttgcaatgt ccaccctccc cgttgaagat
      121 gaggagtcct cggagagcag gatggtggtg acattcctca tgtcagctct cgagtccatg
      181 tgtaaagaac tggccaagtc caaagccgaa gtggcctgca ttgcagtgta tgaaacagac
      241 gtgtttgtcg tcggaactga aagaggacgt gcttttgtca ataccagaaa ggattttcaa
      301 aaagattttg taaaatattg tgttgaagaa gaagaaaaag ctgcagagat gcataaaatg
      361 aaatctacaa cccaggcaaa tcggatgagt gtagatgctg tagaaattaa aacactcaga
      421 aaaacagttg aggactattt ctgcttttgc tatgggaaag ctttaggcaa atccacagtg
      481 gtacctgtac catatgagaa gatgctgcga gaccagtcgg ctgtggtagt gcaggggctt
      541 ccggaaggtg ttgcctttaa acaccccgag aactatgatc ttgcaaccct gaaatggatt
      601 ttggagaaca aagcaggaat ttcattcatc attaagagan cttttttaga gccaaagaag
      661 catgtnntgg tcgtgtgatg gtaacagatg ctgacnggtc natactatct cc
//
