LOCUS       sequence-279761-        2030 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0134
FEATURES             Location/Qualifiers
     source          1..2030
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtccatgg atcagtgaca ttcagggatg tggccattga cttctctcag
       61 gaggagtggg agtgcctgca gcctgatcag aggaccttgt acagggatgt gatgttggag
      121 aactacagcc acctgatctc actggcagga agttccattt ctaaaccaga tgtaattacg
      181 ttactagagc aagagaaaga gccctggatg gttgtaagga aagaaacaag cagacggtat
      241 ccagatttgg agttaaaata tggacctgag aaagtatctc cagaaaatga tacctctgaa
      301 gtaaatttac ccaaacaggt tataaagcaa ataagtacaa ctcttggcat tgaggccttt
      361 tattttagaa atgactcaga atatagacaa tttgagggac tacagggata tcaagaagga
      421 aatatcaatc aaaagatgat cagctatgaa aaactgccta ctcatactcc tcatgcttct
      481 cttatttgca atacacataa accgtatgaa tgtaaggaat gtgggaaata ctttagtcgt
      541 agtgcaaatc ttattcagca tcagagtatt catactggag agaaaccctt tgaatgtaag
      601 gagtgtggga aagcctttcg acttcacata caatttactc gacatcagaa atttcatact
      661 ggtgagaaac cttttgaatg taacgaatgt ggaaaggcct ttagtcttct taccctgctt
      721 aatcgccata agaacattca cacaggtgag aaactgtttg aatgtaagga atgtgggaag
      781 tcctttaatc gtagctcaaa ccttgttcaa catcagagta ttcattctgg tgtaaaacca
      841 tatgaatgta aggagtgtgg gaaaggcttt aatcgtggtg cacaccttat tcagcatcag
      901 aaaattcatt ccaatgagaa accctttgta tgtaaggaat gtgggatggc ctttcgatat
      961 cattaccaac ttattgaaca ttgccaaatt catactggtg agaaaccctt tgaatgtaaa
     1021 gaatgtggaa aggcgtttac tcttctgaca aagcttgttc gacatcagaa gattcatact
     1081 ggtgagaaac cctttgaatg cagggaatgt gggaaggcct ttagtcttct caaccagctt
     1141 aatcgccata agaacattca cacaggtgaa aaaccgtttg aatgtaagga atgtgggaag
     1201 tcctttaatc gtagctcaaa ccttgttcaa catcagagta ttcatgctgg tataaaacca
     1261 tatgaatgta aggagtgtgg gaaaggcttt aatcgtggtg cacaccttat tcagcatcag
     1321 aaaattcatt ccaatgagaa accttttgta tgtagggaat gtgagatggc ctttagatat
     1381 cattgccaac ttattgaaca ttctcgaatt catactggtg acaagccatt tgaatgtcaa
     1441 gactgtggga aggccttcaa tcgtggctca agccttgttc aacatcagag tattcacact
     1501 ggtgagaagc cctatgaatg taaggagtgt gggaaggctt ttagacttta cctacaactt
     1561 tcccaacatc agaaaactca cacaggtgaa aaaccatttg aatgtaagga atgtgggaaa
     1621 ttctttcgtc gtggttcaaa tcttaatcaa catcgaagta ttcatactgg aaagaaaccc
     1681 tttgaatgta aggaatgtgg gaaagccttt cgacttcata tgcaccttat tcgacatcag
     1741 aaattgcata ctggtgagaa accctttgaa tgtaaggagt gtgggaaagc ctttcgactt
     1801 catatgcaac ttattcgaca tcagaaattg catactggtg agaaaccctt tgaatgtaag
     1861 gaatgtggaa aggtttttag tcttcccacc cagcttaatc gccataagaa cattcacaca
     1921 ggtgagaagg catcttagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1981 ctttatatat cttgtggaaa ggacgattcc gccgagatat ctactgtaca
//
