LOCUS       sequence-279767-        3464 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3464)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0137
FEATURES             Location/Qualifiers
     source          1..3464
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggaggc gcctcctgtc tcaggtactt ttaaattcaa tacagatgct
       61 gctgaattca ttcctcagga gaaaaaaaat tctggtctaa attgtgggac tcaaaggaga
      121 ctagactcta ataggattgg tagaagaaat tacagttcac cacctccctg tcacctttcc
      181 aggcaggtcc cttatgatga aatctctgct gttcatcagc atagttatca tccgtcagga
      241 agcaaaccta agagtcagca gacgtctttc cagtcctctc cttgtaataa atcgcccaag
      301 agccatggcc ttcagaatca accttggcag aaattgagga atgagaagca ccatatcaga
      361 gtcaagaaag cacagagtct tgctgagcag acctcagata cagctggatt agagagctcg
      421 accagatcag agagtgggac agacctcaga gagcatagtc cttctgagag tgagaaggaa
      481 gttgtgggtg cagatcccag gggagcaaaa cccaaaaaag caacacagtt tgtatacagc
      541 tatggtagag gaccaaaagt caaggggaaa ctcaaatgtg aatggagtaa ccgaacaact
      601 ccaaaaccgg aggatgctgg acccgaaagt accaaacctg tgggggtttt ccaccctgac
      661 tcttcagagg catcctctag aaaaggagta ttggatgggt atggagccag acgaaatgag
      721 cagagaagat acccacagaa aaggcctccc tgggaagtgg agggggccag gccacgacca
      781 ggcagaaatc caccaaaaca ggagggccac cgacatacaa acgcaggaca cagaaacaac
      841 atgggcccca ttccaaagga tgacctcaat gaaagaccag caaaatctac ctgtgacagt
      901 gagaacttgg cagtcatcaa caagtcttcc aggagggttg accaagagaa atgcactgta
      961 cggaggcagg atcctcaagt agtatctcct ttctcccgag gcaaacagaa ccatgtgcta
     1021 aagaatgtgg aaacgcacac aggttctcta attgaacaac taacaacaga aaaatacgag
     1081 tgcatggtgt gctgtgaatt ggttcgtgtc acggccccag tgtggagttg tcagagctgt
     1141 taccatgtgt ttcatttgaa ctgcataaag aaatgggcaa ggtctccagc atctcaagca
     1201 gatggccaga gtggttggag gtgccctgcc tgtcagaatg tttctgcaca tgttcctaat
     1261 acctacactt gtttctgtgg caaggtaaag aatcctgagt ggagcagaaa tgaaattcca
     1321 catagctgtg gtgaggtttg tagaaagaaa cagcctggcc aggactgccc acattcctgt
     1381 aaccttctct gccatccagg accctgccca ccctgccctg cctttatgac aaaaacatgt
     1441 gaatgtggac gaaccaggca cacagttcgc tgtggtcagg ctgtctcagt ccactgttct
     1501 aacccatgtg agaatatttt gaactgtggt cagcaccagt gtgctgagct gtgccatggg
     1561 ggtcagtgcc agccttgcca gatcattttg aaccaggtat gctattgcgg cagcacctcc
     1621 cgagatgtgt tatgtggaac cgatgtagga aagtctgatg gatttgggga tttcagctgt
     1681 ttaaagatat gtggcaagga cttgaaatgc ggtaaccata catgttcgca agtgtgccac
     1741 cctcagccct gccagcaatg cccacggctc ccccagctgg tgcgctgttg cccctgtggc
     1801 caaactcctc tcagccaatt gctagaactt ggaagtagta gtcggaaaac atgcatggac
     1861 cctgtgcctt catgtggaaa agtgtgcggc aagcctctgc cttgtggttc cttagatttc
     1921 attcatacct gtgaaaagct ctgccatgaa ggagactgtg gaccatgctc tcgcacatca
     1981 gttatttcct gcagatgctc tttcagaaca aaggagcttc catgtaccag tctcaaaagt
     2041 gaagatgcta catttatgtg tgacaagcgg tgtaacaaga aacggttgtg tggacggcat
     2101 aaatgtaatg agatatgctg tgtggataag gagcacaagt gtcctttgat ttgtgggagg
     2161 aaactccgtt gtggccttca taggtgtgaa gaaccttgtc atcgtggaaa ctgccagaca
     2221 tgctggcaag ccagttttga tgaattaacc tgccattgtg gtgcatcagt gatttaccct
     2281 ccagttccct gtggtactag gccccctgaa tgtacccaaa cctgcgctag agtccatgag
     2341 tgtgaccatc cagtatatca ttcttgtcat agtgaggaga agtgtccccc ttgcactttc
     2401 ctaactcaga agtggtgcat gggcaagcat gagtttcgga gcaacatccc ctgtcacctg
     2461 gttgatatct cttgcggatt accctgcagt gccacgctac catgtgggat gcacaaatgt
     2521 cagagactct gtcacaaagg ggagtgtctt gtggatgagc cctgcaagca gccctgcacc
     2581 acccccagag ctgactgtgg tcacccgtgt atggcaccct gccataccag ctcaccctgc
     2641 cctgtgactg cttgtaaagc taaggtagag ctacagtgtg aatgtggacg aagaaaagag
     2701 atggtgattt gctctgaagc atctagtact tatcaaagaa tagctgcaat ctccatggcc
     2761 tctaagataa cagacatgca gcttggaggt tcagtggaga tcagcaagtt aattaccaaa
     2821 aaggaagttc atcaagccag gctggagtgt gatgaggagt gttcagcctt ggaaaggaaa
     2881 aagagattag cagaggcatt tcatatcagt gaggattctg atcctttcaa tatacgttct
     2941 tcagggtcaa aattcagtga tagtttgaaa gaagatgcca ggaaggactt aaagtttgtc
     3001 agtgacgttg agaaggaaat ggaaaccctc gtggaggccg tgaataaggg aaagaatagt
     3061 aagaaaagcc acagcttccc tcccatgaac agagaccacc gccggatcat ccatgacttg
     3121 gcccaagttt atggcctgga gagcgtgagc tatgacagtg aaccgaagcg caatgtggtg
     3181 gtcactgcca tcagggggaa gtccgtttgt cctcctacca cgctgacagg tgtgcttgaa
     3241 agggaaatgc aggcacggcc tccaccaccg attcctcatc acagacatca gtcagacaag
     3301 aatcctggga gcagtaattt acagaaaata accaaggagc caataattga ctattttgac
     3361 gtccaggact agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
     3421 atatcttgtg gaaaggacga gtttgccgtt ccgcgacgtt ccta
//
