LOCUS       sequence-279776-        1781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0161
FEATURES             Location/Qualifiers
     source          1..1781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacaatca gtgcaccgtc caggtgaggt tagagctggg gcatcgcgcc
       61 caactgcgca agaagcccac cacggagggg ttcactcacg actggatggt gtttgtccgc
      121 ggccccgagc aatgtgacat ccagcacttc gtggagaagg tggtcttctg gctgcacgac
      181 agcttcccca agcccagacg cgtgtgcaag gagcccccct acaaagtaga ggagtcgggg
      241 tacgctggct tcatcatgcc catcgaggtg cacttcaaaa acaaggagga gccgaggaag
      301 gtctgcttca cctacgacct gttcctgaac ctggaaggca acccgcccgt gaaccacctg
      361 cgctgcgaga agctcacctt caacaacccc accacggagt tccggtacaa gctcctgcgg
      421 gccggcgggg tgatggtaat gcccgaagga gcagacacgg tgtccaggcc cagtcccgac
      481 taccccatgt tacccacaat tccactctct gccttctctg accccaagaa gaccaaacca
      541 tcccacggct ccaaggacgc caacaaggag agcagcaaga cctccaagcc acacaaggtg
      601 accaaggagc accgggagcg gccccgcaaa gactccgaga gcaagagctc ctccaaggag
      661 ctggagcgtg agcaggccaa aagctccaag gacacctcgc ggaagctggg cgagggccgg
      721 ctgcccaagg aggagaaggc gccaccgccc aaggctgcct tcaaggaacc caagatggcc
      781 ctgaaagaga ccaagctgga aagcacgtcc cccaagggtg ggcccccacc cccaccccca
      841 cccccacccc gggcttccag caagcggccg gccaccgccg actcgccaaa gcccagcgcc
      901 aagaagcaga agaagagcag ctcgaagggg tcccggagtg ctccaggcac ctcgccccgc
      961 acctcctcct cctcctcctt ctcggacaag aagccggcca aggacaagag cagcaccaga
     1021 ggggagaagg tgaaggccga gagtgagccc cgggaggcca aaaaggccct ggaggtggag
     1081 gagtccaact cagaggacga ggcctccttc aagtccgagt ctgcccagtc aagcccgtcc
     1141 aactccagct ccagctcaga ctccagctca gactcagact tcgagccatc ccagaaccac
     1201 agccaaggac ccctgcgctc catggtggag gacctgcagt ccgaggagtc cgacgaggac
     1261 gactcttcgt caggcgagga ggctgccggc aagaccaacc cggggaggga ctccaggttg
     1321 agcttcagcg acagcgagag tgacaacagc gccgactcct ccctgcccag ccgtgagccc
     1381 ccaccccccc agaagccacc cccgcccaac agcaaggtgt caggccggag gagccccgag
     1441 tcctgcagca agcctgagaa gatcctcaag aagggcacct acgacaaggc ctacacggat
     1501 gagctggtgg agctacaccg gaggctgatg gcgctgcggg agcgcaacgt gctgcagcag
     1561 attgtgaatc tgatcgagga gactggccac ttcaatgtca ccaacaccac cttcgacttc
     1621 gacctcttct ccctggacga gaccaccgtg cgcaaactgc agagctgcct ggaggccgtg
     1681 gccacatagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1741 tcttgtggaa aggacgacgc agcgtcgcgc ccggagggag t
//
