LOCUS       sequence-279782-        2321 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0165
FEATURES             Location/Qualifiers
     source          1..2321
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctacctt gcctgcggag ccgagcgcgg ggccggcggc tgggggggag
       61 gcggtggcgg cggcggcggc gaccgaagag gaggaggagg aagcgcgcca gctcttgcag
      121 actttgcagg cggccgaggg tgaggcggcg gcggcggccg gggccggggc gggcgcagcg
      181 gctgcgggag ctgagggccc gggatccccg ggcgtccccg ggtcgccccc cgaggccgct
      241 tccgaaccgc ccacgggcct ccgcttctcg cccgagcagg tggcgtgcgt ctgcgaggcg
      301 ctgctccagg cgggccacgc cggccgcttg agccgcttcc tgggcgcact gcccccggcc
      361 gagcgcctac gtggcagcga cccggtgttg cgcgcgcggg ccctggtggc cttccagcgg
      421 ggcgagtacg ccgagctcta ccggctactc gagagccgcc ccttccccgc cgcccaccac
      481 gccttcctgc aggacctcta cctgcgcgcg cgctaccatg aggccgagcg ggcccgcggc
      541 cgcgcgcttg gcgcagtgga caagtatcga ctgcgcaaga agttcccgct gcccaagacc
      601 atctgggacg gcgaggagac agtctactgc ttcaaggagc gctcccgcgc agcgctcaag
      661 gcctgctacc gcggcaaccg ctaccccacg ccggacgaga agcgccgcct ggccacactc
      721 accggcctgt cgctcacgca ggtcagcaac tggttcaaga accggcgaca gcgcgaccgg
      781 accggggccg gaggcggcgc gccctgcaag agcgagtctg atgggaatcc cacgactgag
      841 gacgagtcca gccgaagtcc tgaggacctg gagagagggg cggccccagt gtccgccgag
      901 gccgctgccc agggctccat attcctggca gggaccggcc ctcccgcgcc ttgcccggct
      961 tcctcctcca tcctggtgaa cgggagcttc ctggcagcca gcggctcccc agcagtgctc
     1021 ctcaacgggg gccccgtcat catcaacggc ctggccctgg gcgaggcctc cagcctgggc
     1081 ccgctgctgc tcactggggg cgggggtgcc cctccaccgc agcccagccc tcagggggcc
     1141 agcgagacca agacctctct ggtcctggac cctcagacag gggaggtgcg gctggaggag
     1201 gctcagtcgg aggcccctga gaccaaaggg gcccaggtgg ctgctccggg accagccctt
     1261 ggagaggagg tcctggggcc cctggcccaa gtggtgcctg gccccccgac ggctgccacc
     1321 tttcctctgc ccccggggcc agtgcctgct gtggctgccc cacaagtggt accgctctcc
     1381 ccacccccgg ggtatcccac gggcctgagc cccacctccc cactattgaa cctgccccag
     1441 gtagtaccca cctcacaggt ggtgaccctg ccccaggctg tggggcccct gcagctgttg
     1501 gcagccgggc caggcagccc tgtgaaggtg gcagctgcag caggccctgc caatgtgcac
     1561 ctcatcaact ccggggtggg cgtgactgcc ctgcagctgc cttcggccac tgccccagga
     1621 aacttcctcc tggccaaccc tgtgtctggc agccccatcg tgacgggtgt ggccctgcag
     1681 cagggcaaga tcatcctcac cgccaccttc cccaccagca tgctcgtctc ccaggtcctg
     1741 ccgccagccc ccggcctggc cctgccactg aagccagaga cggccatctc cgtgcctgag
     1801 ggaggcctcc cggtggcccc cagccctgct ctcccagagg ctcacgccct aggcaccctt
     1861 tctgcacagc agccaccccc cgccgctgcc accacctcca gcaccagcct gcccttctcc
     1921 cctgactccc ctggcctcct gcccaacttc ccggcgcccc caccagaggg gctgatgttg
     1981 tcacccgcgg ccgtgcctgt ctggtcagca gggctggaac taagcgcagg aacagagggg
     2041 ctgctggaag cggaaaaggg gctggggaca caggcccccc acaccgtgct gaggctgcca
     2101 gaccccgacc ctgaggggct gctcctgggg gccaccgcag ggggtgaggt tgacgagggg
     2161 ttggaagctg aggccaaggt tctgacccag ctccagtcgg tgcctgtgga ggagcccttg
     2221 gaactgtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2281 tcttgtggaa aggacgacat gtagataaag acctccttca t
//
