LOCUS       sequence-27979-e         638 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Cheriyath V, Roy AL
  TITLE     Alternatively spliced isoforms of TFII-I. Complex formation, nuclear
            translocation, and differential gene regulation.
  JOURNAL   J Biol Chem. 2000 Aug 25. 275(34):26300-8.
  PUBMED    10854432
REFERENCE   2  (bases 1 to 638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2000 Aug 25. 275(34):26300-8."
COMMENT     Sequence Label: pEBG-TFII-I gamma
FEATURES             Location/Qualifiers
     source          1..638
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             40..57
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             67..84
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 tcgganctgg ttccgcgtgg anccaccatg ggcagcagcc atcatcatca tcatcacagc
       61 agcggcctgg tgccgcgcgg cagccatatg gcccaagttg caatgtccac cctccccgtt
      121 gaagatgagg agtcctcgga gagcaggatg gtggtgacat tcctcatgtc agctctcgag
      181 tccatgtgta aagaactggc caagtccaaa gccgaagtgg cctgcattgc agtgtatgaa
      241 acagacgtgt ttgtcgtcgg aactgaaaga ggacgtgctt ttgtcaatac cagaaaggat
      301 tttcaaaaag attttgtaaa atattgtgtt gaagaagaag aaaaagctgc agagatgcat
      361 aaaatgaaat ctacaaccca ggcaaatcgg atgagtgtag atgctgtaga aattgaaaca
      421 ctcagaaaaa cagttgagga ctatttctgc ttttgctatg ggaaagcttt aggcaaatcc
      481 acagtggtac ctgtaccata tgagaagatg ctgcgagacc agtcggctgt ggtagtgcag
      541 gggcttccgg aaggtgttgc ctttaaacac cccgagaact atgatcttgc aaccctgaaa
      601 tggattttgg agaacaaagc angaatttca ttncatca
//
