LOCUS       sequence-279791-        1616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0172
FEATURES             Location/Qualifiers
     source          1..1616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             538..561
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 accgccacca tgaaggctgc ctacaccgcc tatcgatgcc tcaccaaaga cctagaaggc
       61 tgcgccatga acccggagct gacaatggaa agtctgggca ctttgcacgg gccggccggc
      121 ggcggcagtg gcgggggcgg cggcgggggc ggcgggggcg gcggcggggg cccgggccat
      181 gagcaggagc tgctggccag ccccagcccc caccacgcgg gccgcggcgc cgctggctcg
      241 ctgcggggcc ctccgccgcc tccaaccgcg caccaggagc tgggcacggc ggcagcggcg
      301 gcagcggcgg cgtcgcgctc ggccatggtc accagcatgg cctcgatcct ggacggcggc
      361 gactaccggc ccgagctctc catcccgctg caccacgcca tgagcatgtc ctgcgactcg
      421 tctccgcctg gcatgggcat gagcaacacc tacaccacgc tgacaccgct ccagccgctg
      481 ccacccatct ccaccgtgtc tgacaagttc caccaccctc acccgcacca ccatccgcac
      541 caccaccacc accaccacca ccagcgcctg tccggcaacg tcagcggcag cttcaccctc
      601 atgcgcgacg agcgcgggct cccggccatg aacaacctct acagtcccta caaggagatg
      661 cccggcatga gccagagcct gtccccgctg gccgccacgc cgctgggcaa cgggctaggc
      721 ggcctccaca acgcgcagca gagtctgccc aactacggtc cgccgggcca cgacaaaatg
      781 ctcagcccca acttcgacgc gcaccacact gccatgctga cccgcggtga gcaacacctg
      841 tcccgcggcc tgggcacccc acctgcggcc atgatgtcgc acctgaacgg cctgcaccac
      901 ccgggccaca ctcagtctca cgggccggtg ctggcaccca gtcgcgagcg gccaccctcg
      961 tcctcatcgg gctcgcaggt ggccacgtcg ggccagctgg aagaaatcaa caccaaagag
     1021 gtggcccagc gcatcacagc ggagctgaag cgctacagta tcccccaggc gatctttgcg
     1081 cagagggtgc tgtgccggtc tcaggggact ctctccgacc tgctccggaa tccaaaaccg
     1141 tggagtaaac tcaaatctgg cagggagacc ttccgcagga tgtggaagtg gcttcaggag
     1201 cccgagttcc agcgcatgtc cgccttacgc ctggcagcgt gcaaacgcaa agagcaagaa
     1261 ccaaacaaag acaggaacaa ttcccagaag aagtcccgcc tggtgttcac tgacctccaa
     1321 cgccgaacac tcttcgccat cttcaaggag aacaaacgcc cgtcaaagga gatgcagatc
     1381 accatttccc agcagctggg cctggagctc acaaccgtca gcaacttctt catgaacgcc
     1441 cggcgccgca gcctggagaa gtggcaagac gatctgagca cagggggctc ctcgtccacc
     1501 tccagcacgt gtaccaaagc atagtaatga actagtccgt aacttgaaag tatttcgatt
     1561 tcttggcttt atatatcttg tggaaaggac gattacaggc atgctctagc cctaag
//
