LOCUS       sequence-279818-        1907 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0216
FEATURES             Location/Qualifiers
     source          1..1907
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             580..588
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tgatgcaaga atctgggact gagacaaaaa gtaacggttc agccatccag
       61 aatgggtcgg gcggcagcaa ccacttacta gagtgcggcg gtcttcggga ggggcggtcc
      121 aacggagaga cgccggccgt ggacatcggg gcagctgacc tcgcccacgc ccagcagcag
      181 cagcaacagg cacttcaggt ggcaagacag ctccttcttc agcagcaaca gcagcagcaa
      241 gttagtggat taaaatctcc caagaggaat gacaaacaac cagctcttca gcaacagcag
      301 gtggctaccc agcagttggc ttttcagcag cagcttttac agatgcagca gttacagcag
      361 cagcacctcc tgtctttgca gcgccaaggc cttctgacaa ttcagcccgg gcagcctgcc
      421 cttccccttc aacctcttgc tcaaggcatg attccaacag aactgcagca gctctggaaa
      481 gaagtgacaa gtgctcatac tgcagaagaa accacaggca acaatcacag cagtttggat
      541 ctgaccacga catgtgtctc ctcctctgca ccttccaaga cctccttaat aatgaaccca
      601 catgcctcta ccaatggaca gctctcagtc cacactccca aaagggaaag tttgtcccat
      661 gaggagcacc cccatagcca tcctctctat ggacatggtg tatgcaagtg gccaggctgt
      721 gaagcagtgt gcgaagattt ccaatcattt ctaaaacatc tcaacagtga gcatgcgctg
      781 gacgatagaa gtacagccca atgtagagta caaatgcagg ttgtacagca gttagagcta
      841 cagcttgcaa aagacaaaga acgcctgcaa gccatgatga cccacctgca tgtgaagtct
      901 acagaaccca aagccgcccc tcagcccttg aatctggtat caagtgtcac tctctccaag
      961 tccgcatcgg aggcttctcc acagagctta cctcatactc caacgacccc aaccgccccc
     1021 ctgactcccg tcacccaagg cccctctgtc atcacaacca ccagcatgca cacggtggga
     1081 cccatccgca ggcggtactc agacaaatac aacgtgccca tttcgtcagc agatattgcg
     1141 cagaaccaag aattttataa gaacgcagaa gttagaccac catttacata tgcatcttta
     1201 attaggcagg ccattctcga atctccagaa aagcagctaa cactaaatga gatctataac
     1261 tggttcacac gaatgtttgc ttacttccga cgcaacgcgg ccacgtggaa gaatgcagtg
     1321 cgtcataatc ttagtcttca caagtgtttt gtgcgagtag aaaacgttaa aggggcagta
     1381 tggacagtgg atgaagtaga attccaaaaa cgaaggccac aaaagatcag tggtaaccct
     1441 tcccttatta aaaacatgca gagcagccac gcctactgca cacctctcaa tgcagcttta
     1501 caggcttcaa tggctgagaa tagtatacct ctatacacta ccgcttccat gggaaatccc
     1561 actctgggca acttagccag cgcaatacgg gaagagctga acggggcaat ggagcatacc
     1621 aacagcaacg agagtgacag cagtccaggc agatctccta tgcaagccgt gcatcctgta
     1681 cacgtcaaag aagagcccct cgatccagag gaagctgaag ggcccctgtc cttagtgaca
     1741 acagccaacc acagtccaga ttttgaccat gacagagatt acgaagatga accagtaaac
     1801 gaggacatgg agtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1861 tatatatctt gtggaaagga cgaagcttgt tggctgctta atgcagt
//
