LOCUS       sequence-279821-        2141 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0217
FEATURES             Location/Qualifiers
     source          1..2141
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             814..822
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 accgccacca tgttccagtg tgttttctct tcgagtgtgc ttcagccgca cagtacttcc
       61 tgtttattca aacatctttt ttaccactcg gcaacccccg cttcgcagaa acagccagaa
      121 cccatctaca gcaagaagac ggaaatccaa aggcagacag tacgggctcc cttcgccaaa
      181 ctcttcattt tctctgcact tcaggtggca agacagctcc ttcttcagca gcaacagcag
      241 cagcaagtta gtggattaaa atctcccaag aggaatgaca aacaaccagc tcttcaggtt
      301 cccgtgtcag tggctatgat gacacctcaa gttatcactc cccagcaaat gcagcagatc
      361 ctccagcaac aagtgctgag ccctcagcag ctccaggttc tcctccagca gcagcaggcc
      421 ctcatgcttc aacagcagca gcttcaagag ttttataaaa aacaacagga acagttgcag
      481 cttcaacttt tacaacaaca acatgctgga aaacagccta aagagcaaca gcaggtggct
      541 acccagcagt tggcttttca gcagcagctt ttacagatgc agcagttaca gcagcagcac
      601 ctcctgtctt tgcagcgcca aggccttctg acaattcagc ccgggcagcc tgcccttccc
      661 cttcaacctc ttgctcaagg catgattcca acagaactgc agcagctctg gaaagaagtg
      721 acaagtgctc atactgcaga agaaaccaca ggcaacaatc acagcagttt ggatctgacc
      781 acgacatgtg tctcctcctc tgcaccttcc aagacctcct taataatgaa cccacatgcc
      841 tctaccaatg gacagctctc agtccacact cccaaaaggg aaagtttgtc ccatgaggag
      901 cacccccata gccatcctct ctatggacat ggtgtatgca agtggccagg ctgtgaagca
      961 gtgtgcgaag atttccaatc atttctaaaa catctcaaca gtgagcatgc gctggacgat
     1021 agaagtacag cccaatgtag agtacaaatg caggttgtac agcagttaga gctacagctt
     1081 gcaaaagaca aagaacgcct gcaagccatg atgacccacc tgcatgtgaa gtctacagaa
     1141 cccaaagccg cccctcagcc cttgaatctg gtatcaagtg tcactctctc caagtccgca
     1201 tcggaggctt ctccacagag cttacctcat actccaacga ccccaaccgc ccccctgact
     1261 cccgtcaccc aaggcccctc tgtcatcaca accaccagca tgcacacggt gggacccatc
     1321 cgcaggcggt actcagacaa atacaacgtg cccatttcgt cagcagatat tgcgcagaac
     1381 caagaatttt ataagaacgc agaagttaga ccaccattta catatgcatc tttaattagg
     1441 caggccattc tcgaatctcc agaaaagcag ctaacactaa atgagatcta taactggttc
     1501 acacgaatgt ttgcttactt ccgacgcaac gcggccacgt ggaagaatgc agtgcgtcat
     1561 aatcttagtc ttcacaagtg ttttgtgcga gtagaaaacg ttaaaggggc agtatggaca
     1621 gtggatgaag tagaattcca aaaacgaagg ccacaaaaga tcagtggtaa cccttccctt
     1681 attaaaaaca tgcagagcag ccacgcctac tgcacacctc tcaatgcagc tttacaggct
     1741 tcaatggctg agaatagtat acctctatac actaccgctt ccatgggaaa tcccactctg
     1801 ggcaacttag ccagcgcaat acgggaagag ctgaacgggg caatggagca taccaacagc
     1861 aacgagagtg acagcagtcc aggcagatct cctatgcaag ccgtgcatcc tgtacacgtc
     1921 aaagaagagc ccctcgatcc agaggaagct gaagggcccc tgtccttagt gacaacagcc
     1981 aaccacagtc cagattttga ccatgacaga gattacgaag atgaaccagt aaacgaggac
     2041 atggagtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2101 tcttgtggaa aggacgaaat caaattactg tcgagcatgc t
//
