LOCUS       sequence-279839-        1307 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1307)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0233
FEATURES             Location/Qualifiers
     source          1..1307
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaaaaatc caaaaatttc cgcatcgacg ccctgctggc ggtggacccc
       61 ccacgagccg cctctgcgca gagcgcgccg ctggccttgg tcacgtcgct cgccgccgcc
      121 gcatctggca ccggaggtgg cggcggcggc ggcggggcga gcggcgggac tagcggcagc
      181 tgcagccccg cgtcctcgga gccgccggct gcgcccgccg accgcctgcg cgccgagagc
      241 ccgtcgccgc cgcgcctgct ggccgcgcac tgcgcgctgc tgcccaagcc gggcttcctg
      301 ggcgcgggcg gcggcggcgg cggcacgggc ggcgggcacg gggggcccca ccaccacgcg
      361 catccgggcg cagcggccgc tgccgccgcc gccgccgccg ccgccgccgc tgggggcctg
      421 gcgctggggc tgcaccctgg gggcgcgcag ggcggcgcgg gcctcccggc gcaggcggcg
      481 ctctacggcc acccggtcta cggctactcc gcggcggcgg cggcggctgc gctggcgggc
      541 cagcacccgg cgctctccta ctcgtacccg caggtgcaag gcgcgcaccc cgcgcacccc
      601 gccgacccca tcaagctggg cgccggcacc ttccagctgg accagtggct gcgcgcgtcc
      661 accgcgggca tgatcctgcc taagatgccc gacttcaact cccaggcgca gtcgaacctc
      721 ctggggaagt gccgccggcc gcgcaccgcc ttcaccagcc agcagctgct ggagctggag
      781 caccagttca agctcaacaa gtacctgtcg cggcccaagc gcttcgaggt ggccacctcg
      841 ctcatgctca ccgagaccca ggtgaagatt tggttccaga accggcggat gaaatggaaa
      901 cgcagcaaaa aggccaaaga gcaggcggcg caggaagcgg agaaacagaa gggcggcggc
      961 gggggcgcgg ggaagggcgg cgcggaggag ccgggagccg aggagctgct ggggccgcca
     1021 gcgcccggag acaagggcag cggacgccgc ctgcgggact tgagggacag tgaccccgag
     1081 gaggacgagg acgaggacga cgaggaccat ttcccctaca gcaacggcgc cagcgtccac
     1141 gccgcctcct ccgactgctc ctcggaggac gactcgccgc ccccgcggcc cagccaccag
     1201 cccgcgcccc agtagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1261 tatatatctt gtggaaagga cgatgcgttc gctaatccga tagcgcc
//
