LOCUS       sequence-279842-        2480 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0241
FEATURES             Location/Qualifiers
     source          1..2480
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(1575..1586)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(1743..1754)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tggcagccat caacccgtgg gcctcctggg gtgcccttac ggaccaatct
       61 tgggggatga cagctgttga cccatgggcc tcctgggctc tgtgtcctca gtatcctgcc
      121 tggcacgtgg agggaagcct ggaggagggg aggagggcca ctgggctccc agcagcccag
      181 gttcaggaac cagtgacctt caaggacgtg gccgtggact tcacccaaga agagtggggg
      241 cagctggacc ttgttcagag gaccctgtac cgtgatgtga tgctggagac ctatggtcac
      301 ctgctctctg tgggaaatca gattgccaag cctgaggtca tctccctgtt ggagcaagga
      361 gaagagccgt ggtcagtgga gcaggcatgt cctcaacgca cttgtccaga atgggtgaga
      421 aatcttgaaa gcaaagcatt gatcccagca cagagcattt ttgaggaaga acaatcccat
      481 ggcatgaagt tggaaagata tatatgggat gatccttggt tctccaggtt agaagttttg
      541 ggatgtaaag accaattaga aatgtaccac atgaaccaga gtacagctat gaggcagatg
      601 gtcttcatgc aaaagcaagt actatcccag agaagctctg aattctgtgg acttggggca
      661 gagtttagcc agaacttaaa ctttgttcca tctcagagag tttctcagat agaacatttc
      721 tataagcctg atacacatgc tcaaagttgg agatgtgact cagccataat gtatgcagat
      781 aaggttacct gtgaaaataa tgattatgac aaaactgttt atcagtccat tcaacctatt
      841 taccctgcaa gaatacaaac tggagataat cttttcaaat gtactgatgc tgttaaatct
      901 ttcaatcata taatacattt tggtgatcat aaaggaattc acacaggaga aaaactctat
      961 gaatataagg aatgccatca aatctttaac cagagcccat catttaatga acacccaagg
     1021 cttcatgttg gagaaaacca gtataattac aaagaatatg agaatatctt ttatttctca
     1081 tcctttatgg aacatcaaaa aattggtact gtagagaaag cgtataaata caatgaatgg
     1141 gagaaagtct ttgggtatga ctctttcctt actcaacata caagcactta cactgcagag
     1201 aaaccctatg actacaatga atgtgggacg tctttcatct ggagctctta ccttattcaa
     1261 cataagaaaa ctcatactgg agaaaaaccc tatgaatgtg ataaatgtgg aaaagttttt
     1321 aggaatcgct cagcccttac gaaacatgaa cggactcaca ctggaataaa accctatgaa
     1381 tgtaataaat gtggaaaagc cttcagctgg aattctcatc ttattgtaca taagagaatt
     1441 catacaggag aaaaacctta tgtttgtaat gagtgtggga aatctttcaa ctggaactct
     1501 catcttattg gacatcagag gactcataca ggagagaaac cttttgaatg tactgaatgt
     1561 gggaaatcat tcagctggag ctcccatctt attgcccata tgagaatgca tactggagag
     1621 aaacccttta aatgtgatga atgtgaaaaa gcttttaggg actactcagc ccttagtaaa
     1681 catgaaagaa ctcattctgg agcaaaacca tataaatgta ctgaatgtgg aaaatccttc
     1741 agctggagct cccatcttat tgcccatcag agaactcaca cgggagagaa accatataac
     1801 tgtcaggaat gtggcaaagc attcagagaa cgctcagccc tcactaaaca tgagataatt
     1861 cattctggaa ttaagcccta tgaatgtaat aaatgtggaa aatcctgtag ccagatggct
     1921 caccttgtta gacatcaaag gactcatact ggagaaaaac cctatgaatg caataaatgt
     1981 ggaaaatcct tcagtcagag ctgtcacctt gttgctcatc ggagaattca cactggtgag
     2041 aaaccctata aatgtaatca gtgtgaaaga tcctttaact gtagttctca cctcattgca
     2101 caccggagaa ctcatactgg agagaaacca tacaggtgta atgaatgtgg gaaagcattt
     2161 aatgagagtt catcccttat tgtacaccta agaaaccata ctggagaaaa gccctacaaa
     2221 tgtaatcatt gtgaaaaagc attttgtaag aattcttccc ttattattca tcagagaatg
     2281 catagtggag agaaacgctt tatatgcagt gaatgtggaa aagcctttag tggtcactca
     2341 gccctacttc aacaccagag aaatcacagt gaagagaaac tgaattagta atgaactagt
     2401 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgattat
     2461 cctccattat tcgcgagtcc
//
