LOCUS       sequence-279845-        3695 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0245
FEATURES             Location/Qualifiers
     source          1..3695
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccgctgc cgccgtcacc cgcgggaccc cgggagaaaa cagccaccac
       61 ctgaagatct ttctccccaa gaagctgctg gagtgtcttc ctcgctgccc gctgctgcct
      121 ccagagaggc tacggtggaa tacaaatgag gagattgcat cctacctgat cacctttgag
      181 aagcatgatg agtggctgtc ttgtgcccca aagacaaggc ctcagaatgg ctccatcatc
      241 ctctacaatc gcaagaaggt gaaatatcgg aaggatggtt acctctggaa gaagcggaag
      301 gatgggaaga ccacccgaga ggaccacatg aagctgaagg tccagggcat ggagtgtctc
      361 tatggctgct acgttcactc ttccatcgtc cccacattcc atcggcgctg ctactggctg
      421 ctccagaacc ctgacatcgt ccttgtgcac tacctgaacg tcccagccct ggaggactgt
      481 ggaaagggct gcagccccat cttttgttcc atcagcagcg accgtcgaga gtggctgaag
      541 tggtcccggg aggagttgtt gggacagctg aagcccatgt ttcatggcat caagtggagc
      601 tgcgggaatg gaacagaaga gttctctgta gaacacctgg tgcagcagat tttggacacc
      661 cacccaacca agcctgctcc ccgaacccac gcctgtctct gcagtggggg gcttggttct
      721 gggagcctta cccacaaatg cagcagcacg aaacaccgca tcatctctcc caaagtggag
      781 ccccgagctt taaccctgac ctctatcccc cacgctcacc ccccagagcc tcctccactg
      841 atagccccac ttcccccaga gctccccaag gcacacacct ccccatcttc ttcctcttct
      901 tcctcctcat caggttttgc agagccccta gaaatcagac ctagccctcc cacttctcga
      961 gggggttctt caagaggagg cactgctatc ctcctcctga caggactgga gcagcgggct
     1021 ggaggcttga cgcccaccag gcacttggct ccacaggctg atcctaggcc ttccatgagt
     1081 ttggcagtgg ttgtaggcac tgagccttct gccccaccag ctcctcccag tcctgccttt
     1141 gaccctgatc gttttctcaa cagcccccag aggggccaga catatggagg ggggcaggga
     1201 gtaagcccag acttccccga ggcagaggcc gctcataccc cctgttctgc cctagagcct
     1261 gctgctgccc tggagcccca ggcagctgct cggggtcccc caccacagtc agtagcaggt
     1321 gggagaagag gaaactgctt cttcatccaa gatgatgaca gtggggagga gctcaagggt
     1381 cacggggctg ccccacccat accttcaccc cctccctcac ccccaccctc acctgccccc
     1441 ttggagccgt caagcagggt aggaagagga gaggccttgt ttggaggacc tgttggggcc
     1501 agtgaactgg agcccttcag tctttcatca ttcccagacc ttatgggaga actcatcagt
     1561 gacgaagctc caagcatccc tgctccgacc ccccagctgt ctcctgctct tagcaccatc
     1621 acagacttct ccccagagtg gtcctaccca gagggtgggg tcaaggtgct catcacaggt
     1681 ccttggaccg aagccgccga gcattactcc tgtgtctttg atcacatcgc agtgccagcc
     1741 tcacttgtcc agcctggtgt cttacgctgc tactgtcccg cccatgaggt agggctggtg
     1801 tctttgcagg tggcagggcg ggaggggccc ctttctgctt ctgtgctctt tgagtatcga
     1861 gcccgccgat tcctgtctct gcctagtact caacttgact ggctgtcact ggacgacaac
     1921 cagttccgga tgtccatact agagcgactg gagcagatgg agaagcggat ggcagagatc
     1981 gcagcagctg ggcaggtgcc ttgccagggt cctgatgctc ctccagttca ggatgaaggc
     2041 caggggcctg ggttcgaagc acgggtagtg gtcttggtag aaagcatgat cccacgctcc
     2101 acctggaagg gtcctgaacg tctggcccat ggaagcccct tccggggcat gagccttctg
     2161 cacctggctg ctgcccaggg ctatgcccgc ctcatcgaga ccctgagcca gtggcggagt
     2221 gtggagactg gaagcttgga cttagagcag gaggttgacc cgctcaacgt ggatcatttc
     2281 tcttgcaccc ctctgatgtg ggcttgtgcc ctgggacacc tggaagctgc tgtgctcctt
     2341 ttccgttgga accgacaggc actgagcatt cccgactctc tgggccgtct gccattgtct
     2401 gtggctcatt cccggggtca tgtgcgcctt gcccgctgcc ttgaggaact acagagacag
     2461 gagccttcgg tggagccccc atttgcccta tcgccaccct cctccagccc agacactggt
     2521 ctgagcagcg tctcctcgcc ctcggagctg tcggatggca ccttttccgt cacgtcagcc
     2581 tattctagtg ccccagatgg cagtcccccc cctgcacctc tgccagcctc tgagatgact
     2641 atggaggaca tggccccagg ccagctttcc tctggtgtcc cagaagcccc cctactcctc
     2701 atggactatg aggctaccaa ctccaagggg cccctctcct cccttcctgc cctcccacca
     2761 gcttcagatg atggggctgc tccagaggac gctgacagcc cacaggctgt ggatgtgatc
     2821 ccggtggaca tgatctcact agccaagcag atcatcgaag ccacaccgga gcggattaaa
     2881 cgagaggact tcgtggggct gcccgaggct ggagcctcaa tgcgggagcg gacaggggct
     2941 gtggggctca gtgagaccat gtcctggctg gccagctacc tggagaatgt ggaccatttc
     3001 cccagctcaa cccctcccag cgaactgccc tttgagcgag gtcgcctggc tgtcccttca
     3061 gcaccctcct gggcagagtt tctctctgca tccaccagtg gcaagatgga aagtgatttt
     3121 gccctgctga cactatcaga tcacgagcag cgggaactgt atgaggctgc ccgagtcatc
     3181 cagacggcct tccgaaagta caagggccgg cggctgaagg agcagcagga ggtagcagca
     3241 gctgtaatcc agcgctgtta ccggaagtac aagcagtttg cactctataa gaagatgacc
     3301 caggcggcca tcctgatcca gagcaagttc cgaagctact atgaacagaa gcgatttcag
     3361 cagagccgcc gagcggctgt gctcatccag cagcactacc gctcctaccg ccgcaggccc
     3421 ggccctcccc accggacttc ggccaccctg cctgcccgca acaaaggctc ctttctcacc
     3481 aagaagcagg accaggcagc ccggaagatc atgagattcc tgcggcgctg ccgacacagg
     3541 atgagggaac tgaagcagaa ccaggagctg gaagggcttc cccagccggg actggccaca
     3601 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     3661 ggaaaggacg atgagcaacc ggcaacctat tgcat
//
