LOCUS       sequence-279857-        3707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0249
FEATURES             Location/Qualifiers
     source          1..3707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggcacaga ctccccctcc ccccggcccc tcaggccggg ggtgaccttg
       61 ccccctggag ccctcaccat gaataccaag gacaccaccg aggttgctga aaacagccac
      121 cacctgaaga tctttctccc caagaagctg ctggagtgtc ttcctcgctg cccgctgctg
      181 cctccagaga ggctacggtg gaatacaaat gaggagattg catcctacct gatcaccttt
      241 gagaagcatg atgagtggct gtcttgtgcc ccaaagacaa ggcctcagaa tggctccatc
      301 atcctctaca atcgcaagaa ggtgaaatat cggaaggatg gttacctctg gaagaagcgg
      361 aaggatggga agaccacccg agaggaccac atgaagctga aggtccaggg catggagaac
      421 cctgacatcg tccttgtgca ctacctgaac gtcccagccc tggaggactg tggaaagggc
      481 tgcagcccca tcttttgttc catcagcagc gaccgtcgag agtggctgaa gtggtcccgg
      541 gaggagttgt tgggacagct gaagcccatg tttcatggca tcaagtggag ctgcgggaat
      601 ggaacagaag agttctctgt agaacacctg gtgcagcaga ttttggacac ccacccaacc
      661 aagcctgctc cccgaaccca cgcctgtctc tgcagtgggg ggcttggttc tgggagcctt
      721 acccacaaat gcagcagcac gaaacaccgc atcatctctc ccaaagtgga gccccgagct
      781 ttaaccctga cctctatccc ccacgctcac cccccagagc ctcctccact gatagcccca
      841 cttcccccag agctccccaa ggcacacacc tccccatctt cttcctcttc ttcctcctca
      901 tcaggttttg cagagcccct agaaatcaga cctagccctc ccacttctcg agggggttct
      961 tcaagaggag gcactgctat cctcctcctg acaggactgg agcagcgggc tggaggcttg
     1021 acgcccacca ggcacttggc tccacaggct gatcctaggc cttccatgag tttggcagtg
     1081 gttgtaggca ctgagccttc tgccccacca gctcctccca gtcctgcctt tgaccctgat
     1141 cgttttctca acagccccca gaggggccag acatatggag gggggcaggg agtaagccca
     1201 gacttccccg aggcagaggc cgctcatacc ccctgttctg ccctagagcc tgctgctgcc
     1261 ctggagcccc aggcagctgc tcggggtccc ccaccacagt cagtagcagg tgggagaaga
     1321 ggaaactgct tcttcatcca agatgatgac agtggggagg agctcaaggg tcacggggct
     1381 gccccaccca taccttcacc ccctccctca cccccaccct cacctgcccc cttggagccg
     1441 tcaagcaggg taggaagagg agaggccttg tttggaggac ctgttggggc cagtgaactg
     1501 gagcccttca gtctttcatc attcccagac cttatgggag aactcatcag tgacgaagct
     1561 ccaagcatcc ctgctccgac cccccagctg tctcctgctc ttagcaccat cacagacttc
     1621 tccccagagt ggtcctaccc agagggtggg gtcaaggtgc tcatcacagg tccttggacc
     1681 gaagccgccg agcattactc ctgtgtcttt gatcacatcg cagtgccagc ctcacttgtc
     1741 cagcctggtg tcttacgctg ctactgtccc gcccatgagg tagggctggt gtctttgcag
     1801 gtggcagggc gggaggggcc cctttctgct tctgtgctct ttgagtatcg agcccgccga
     1861 ttcctgtctc tgcctagtac tcaacttgac tggctgtcac tggacgacaa ccagttccgg
     1921 atgtccatac tagagcgact ggagcagatg gagaagcgga tggcagagat cgcagcagct
     1981 gggcaggtgc cttgccaggg tcctgatgct cctccagttc aggatgaagg ccaggggcct
     2041 gggttcgaag cacgggtagt ggtcttggta gaaagcatga tcccacgctc cacctggaag
     2101 ggtcctgaac gtctggccca tggaagcccc ttccggggca tgagccttct gcacctggct
     2161 gctgcccagg gctatgcccg cctcatcgag accctgagcc agtggcggag tgtggagact
     2221 ggaagcttgg acttagagca ggaggttgac ccgctcaacg tggatcattt ctcttgcacc
     2281 cctctgatgt gggcttgtgc cctgggacac ctggaagctg ctgtgctcct tttccgttgg
     2341 aaccgacagg cactgagcat tcccgactct ctgggccgtc tgccattgtc tgtggctcat
     2401 tcccggggtc atgtgcgcct tgcccgctgc cttgaggaac tacagagaca ggagccttcg
     2461 gtggagcccc catttgccct atcgccaccc tcctccagcc cagacactgg tctgagcagc
     2521 gtctcctcgc cctcggagct gtcggatggc accttttccg tcacgtcagc ctattctagt
     2581 gccccagatg gcagtccccc ccctgcacct ctgccagcct ctgagatgac tatggaggac
     2641 atggccccag gccagctttc ctctggtgtc ccagaagccc ccctactcct catggactat
     2701 gaggctacca actccaaggg gcccctctcc tcccttcctg ccctcccacc agcttcagat
     2761 gatggggctg ctccagagga cgctgacagc ccacaggctg tggatgtgat cccggtggac
     2821 atgatctcac tagccaagca gatcatcgaa gccacaccgg agcggattaa acgagaggac
     2881 ttcgtggggc tgcccgaggc tggagcctca atgcgggagc ggacaggggc tgtggggctc
     2941 agtgagacca tgtcctggct ggccagctac ctggagaatg tggaccattt ccccagctca
     3001 acccctccca gcgaactgcc ctttgagcga ggtcgcctgg ctgtcccttc agcaccctcc
     3061 tgggcagagt ttctctctgc atccaccagt ggcaagatgg aaagtgattt tgccctgctg
     3121 acactatcag atcacgagca gcgggaactg tatgaggctg cccgagtcat ccagacggcc
     3181 ttccgaaagt acaagggccg gcggctgaag gagcagcagg aggtagcagc agctgtaatc
     3241 cagcgctgtt accggaagta caagcagctg acctggattg cacttaagtt tgcactctat
     3301 aagaagatga cccaggcggc catcctgatc cagagcaagt tccgaagcta ctatgaacag
     3361 aagcgatttc agcagagccg ccgagcggct gtgctcatcc agcagcacta ccgctcctac
     3421 cgccgcaggc ccggccctcc ccaccggact tcggccaccc tgcctgcccg caacaaaggc
     3481 tcctttctca ccaagaagca ggaccaggca gcccggaaga tcatgagatt cctgcggcgc
     3541 tgccgacaca ggatgaggga actgaagcag aaccaggagc tggaagggct tccccagccg
     3601 ggactggcca catagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     3661 tatatatctt gtggaaagga cgaggtattc actcacgcgt cgtttga
//
