LOCUS       sequence-279860-        1640 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1640)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0251
FEATURES             Location/Qualifiers
     source          1..1640
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagccca tgaagcttcc catcaccaac agcaggcagc acagaacagc
       61 ttgctgcccc tcctgagctc tgccgtggag ccccctgatc agaaaccatt gcttccaata
      121 ccaataactc agaaacctca gggtgcacca gaaacattaa aggatgccat tgggattaaa
      181 aaagaaaaac ccaaaacttc atttgtgtgc acttactgca gtaaagcttt cagggacagc
      241 tatcacctga ggcgccacga atcctgccac acagggatca agttggtgtc ccggccaaag
      301 aaaaccccca ccacggtggt tccccttatc tctaccatcg ctggggacag cagccgaact
      361 tcgttggtct cgaccattgc aggcatcttg tcaacagtca ctacatcttc ctcgggcacc
      421 aaccccagta gcagtgccag caccacagct atgccagtga cccagtctgt caagaaaccc
      481 agtaagcctg tcaagaagaa ccatgcttgt gagatgtgtg ggaaggcctt ccgagatgtg
      541 taccatctca atcgacacaa gctctcccat tcagatgaga aaccctttga gtgtcctatt
      601 tgtaatcagc gcttcaagag gaaggaccgg atgacttacc atgtgaggtc tcatgaagga
      661 ggcatcacca aaccctatac ttgcagtgtt tgtgggaaag gcttctcaag gcctgaccac
      721 ttaagctgtc atgtaaaaca tgtccattca acagaaagac ccttcaaatg ccaaacgtgc
      781 actgctgcct ttgccaccaa agacagactg cggacacaca tggtgcgcca tgaaggcaag
      841 gtatcatgta acatctgtgg gaagctcctg agtgcagcat acatcaccag ccacttaaag
      901 actcatgggc agagccaaag tatcaactgt aatacatgta aacaaggcat cagtaaaaca
      961 tgcatgagtg aagagaccag taaccaaaag cagcagcagc agcagcagca gcagcagcag
     1021 cagcagcaac aacaacaaca acaacatgtg acaagctggc cagggaagca agtagaaaca
     1081 ctgagactgt gggaagaagc tgttaaagca aggaagaaag aagctgctaa cctgtgccaa
     1141 acctccacgg ctgctacgac acctgtgact ctcactactc cattcagtat aacatcctct
     1201 gtgtcgtctg ggactatgtc aaacccagtc acagtggcag ctgcaatgag catgagaagt
     1261 ccagtaaatg tttcaagtgc agttaacata accagcccaa tgaacatagg gcatcctgta
     1321 actataacca gtccattatc catgacctct cctttaacac tcactacccc agtcaacctc
     1381 cccacccccg tcactgcccc agtgaatata gcacaccctg tcaccatcac atctccaatg
     1441 aatctaccca cacctatgac attagccgcc cctctcaata tagcaatgag acctgtagag
     1501 agcatgcctt tcttgcccca agctttgcct acatcaccgc cttggtagta atgaactagt
     1561 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaatca
     1621 ctagttcctt gcgatgcatg
//
