LOCUS       sequence-279872-        1979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0266
FEATURES             Location/Qualifiers
     source          1..1979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaagagga cagcagagat gtagaagaca gaagtagctc agggtcctgg
       61 gggaatggag gacatccaag cccgtccagg aactatggag atgggactcc ctatgaccac
      121 atgaccagca gggaccttgg gtcacatgac aatctctctc caccttttgt caattccaga
      181 atacaaagta aaacagaaag gggctcatac tcatcttatg ggagagaatc aaacttacag
      241 ggttgccacc agcagagtct ccttggaggt gacatggata tgggcaaccc aggaaccctt
      301 tcgcccacca aacctggttc ccagtactat cagtattcta gcaataatcc ccgaaggagg
      361 cctcttcaca gtagtgccat ggaggtacag acaaagaaag ttcgaaaagt tcctccaggt
      421 ttgccatctt cagtctatgc tccatcagca agcactgccg actacaatag ggactcgcca
      481 ggctatcctt cctccaaacc agcaaccagc actttcccta gctccttctt catgcaagat
      541 ggccatcaca gcagtgaccc ttggagctcc tccagtggga tgaatcagcc tggctatgca
      601 ggaatgttgg gcaactcttc tcatattcca cagtccagca gctactgtag cctgcatcca
      661 catgaacgtt tgagctatcc atcacactcc tcagcagaca tcaattccag tcttcctccg
      721 atgtccactt tccatcgtag tggtacaaac cattacagca cctcttcctg tacgcctcct
      781 gccaacggga cagacagtat aatggcaaat agaggaagcg gggcagccgg cagctcccag
      841 actggagatg ctctggggaa agcacttgct tcgatctatt ctccagatca cactaacaac
      901 agcttttcat caaacccttc aactcctgtt ggctctcctc catctctctc agcaggcaca
      961 gctgtttggt ctagaaatgg aggacaggcc tcatcgtctc ctaattatga aggaccctta
     1021 cactctttgc aaagccgaat tgaagatcgt ttagaaagac tggatgatgc tattcatgtt
     1081 ctccggaacc atgcagtggg cccatccaca gctatgcctg gtggtcatgg ggacatgcat
     1141 ggaatcattg gaccttctca taatggagcc atgggtggtc tgggctcagg gtatggaacc
     1201 ggccttcttt cagccaacag acattcactc atggtgggga cccatcgtga agatggcgtg
     1261 gccctgagag gcagccattc tcttctgcca aaccaggttc cggttccaca gcttcctgtc
     1321 cagtctgcga cttcccctga cctgaaccca ccccaggacc cttacagagg catgccacca
     1381 ggactacagg ggcagagtgt ctcctctggc agctctgaga tcaaatccga tgacgagggt
     1441 gatgagaacc tgcaagacac gaaatcttcg gaggacaaga aattagatga cgacaagaag
     1501 gatatcaaat caattactag caataatgac gatgaggacc tgacaccaga gcagaaggca
     1561 gagcgtgaga aggagcggag gatggccaac aatgcccgag agcgtctgcg ggtccgtgac
     1621 atcaacgagg ctttcaaaga gctcggccgc atggtgcagc tccacctcaa gagtgacaag
     1681 ccccagacca agctcctgat cctccaccag gcggtggccg tcatcctcag tctggagcag
     1741 caagtccgag aaaggaatct gaatccgaaa gctgcgtgtc tgaaaagaag ggaggaagag
     1801 aaggtgtcct cagagcctcc ccctctctcc ttggccggcc cacaccctgg aatgggagac
     1861 gcatcgaatc acatgggaca gatgtagtaa tgaactagtc cgtaacttga aagtatttcg
     1921 atttcttggc tttatatatc ttgtggaaag gacgatgcgc gtcccgacca gaaagctgc
//
