LOCUS       sequence-279890-        5609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 5609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0278
FEATURES             Location/Qualifiers
     source          1..5609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcaaactc gacggggaag gcgcctccgg acgagcggag aaagggactc
       61 gctttcctgg acgagctgcg gcagttccac cacagcagag ggtcgccttt taaaaaaatc
      121 cctgcggtgg gtgggaagga gctggatctt cacggtctct acaccagagt cactacttta
      181 ggcggattcg cgaaggtttc tgagaagaat cagtggggag aaattgttga agagttcaac
      241 tttcccagaa gttgttctaa cgctgccttt gctttaaaac agtattactt gcgttaccta
      301 gaaaagtacg agaaagttca tcattttggg gaggatgatg atgaggtacc accaggcaat
      361 ccaaagccac agcttcctat tggtgcaatt ccatcttcct acaattacca gcaacacagt
      421 gtgtcggatt atctgcgtca aagttatggg ctgtccatgg actttaattc gccaaatgat
      481 tataataaat tggtgctttc actgttatct ggactcccaa atgaagtgga ctttgctatt
      541 aacgtatgca ctctcctatc aaatgaaagc aagcacgtca tgcaacttga aaaagatcct
      601 aaaatcatca ctttactact tgctaatgcc ggggtgtttg acgacacttt aggatccttt
      661 tccactgtat ttggagaaga atggaaagag aagactgata gagacttcgt taagttttgg
      721 aaagacatcg ttgatgataa tgaagttcgt gacctcattt ctgacagaaa caagtctcat
      781 gaaggtacat caggagaatg gatttgggag tctttatttc atccacctcg aaagctgggc
      841 attaacgata ttgaaggaca gcgggtactt cagattgcag tgattttgag aaatctttcc
      901 tttgaggagg gcaatgttaa gctcttggca gctaatcgta cctgtcttcg tttcctatta
      961 ctttctgcac atagtcattt tatttcttta aggcaattag gccttgacac attaggaaat
     1021 attgcagctg agcttttact ggaccctgtt gatttcaaaa ctactcatct gatgtttcat
     1081 actgttacaa aatgtctaat gtcaagggat agatttttaa agatgagagg catggaaatt
     1141 ttgggaaatc tttgcaaagc agaagataat ggtgttttaa tttgtgaata tgtggatcag
     1201 gattcctaca gagagatcat ttgtcatctc actttacctg atgtgctgct tgtaatctca
     1261 acactcgagg tgctatacat gctcacggaa atgggagatg ttgcttgcac aaaaattgca
     1321 aaagtagaaa agagcataga catgttagtg tgtctggttt ctatggatat tcagatgttt
     1381 ggccctgatg cactagctgc ggtaaaactc attgaacacc caagttccag tcatcaaatg
     1441 ttatctgaaa ttaggccaca agctatagag caagtccaaa cccagactca tgtagcatct
     1501 gccccagctt ccagagcagt tgtagcgcag catgttgctc cacctccagg aatagtggaa
     1561 atagatagtg agaagtttgc ttgtcagtgg ctaaatgctc attttgaagt aaatccagat
     1621 tgttctgttt ctcgagcaga aatgtattct gaatacctct cgacttgcag taaattagct
     1681 cgtggtggaa tcctaacatc aactggattt tataaatgtc ttagaacggt ctttccaaat
     1741 catacagtga agagagtgga ggattccagt agcaatgggc aggcacatat tcatgtggta
     1801 ggagtaaaac ggagggctat accacttccc attcagatgt actatcagca gcaaccagtt
     1861 tctacttctg ttgttcgtgt tgattctgtt cctgatgtat ctcctgctcc ttcacctgca
     1921 ggaatccctc atggatcaca aaccatagga aaccattttc agaggactcc tgttgccaac
     1981 caatcttcaa atctgactgc aacacaaatg tcttttcctg tacaaggtgt tcatactgtg
     2041 gcacaaactg tttcaagaat tccacaaaat ccttcacctc atacccacca gcaacaaaat
     2101 gctccagtga ctgtcattca aagtaaagct ccaattcctt gtgaagttgt taaggctaca
     2161 gttatccaga attccatacc ccagacagga gttcctgtta gtattgctgt tggaggagga
     2221 cctccacaga gttctgttgt tcagaatcat agtacagggc cacaacctgt tacagttgtg
     2281 aattctcaga cattgcttca ccatccatct gtaattccac agcagtctcc attacacaca
     2341 gtggtaccag gacagatccc ttcaggcact cctgttacag taattcaaca agctgtccca
     2401 cagagtcata tgtttggcag agtacagaac ataccagcat gtacttctac agtttcacag
     2461 ggtcaacagt taatcaccac atcaccccaa cctgtgcaaa cttcatctca acagacatca
     2521 gctggtagcc agtcacaaga tactgttatc atagcacccc cacagtatgt aacaacttct
     2581 gcatccaata ttgtctcagc aacttcagta cagaattttc aggtagctac aggacaaatg
     2641 gttactattg ctggtgtccc aagtccacaa gcctcaaggg tagggtttca gaacattgca
     2701 ccaaaacctc tcccttctca gcaagtttca tctacagtgg tacagcagcc tattcaacaa
     2761 ccacagcagc caacccaaca aagcgtagtg attgtaagcc agccagctca acaaggtcaa
     2821 acttatgcac cagccattca ccaaattgtt cttgctaatc cagcagctct tccagctggt
     2881 cagacagttc agctaactgg acaacctaac ataactccat cttcttcacc atcacctgtc
     2941 ccagctacta ataaccaagt ccctactgcc atgtcgtcgt cctctacccc tcaatcacag
     3001 ggaccacctc ctactgtcag tcaaatgtta tctgtgaaaa ggcagcaaca gcagcaacat
     3061 tcaccagcac ccccaccaca gcaggtacaa gtacaagttc agcagcccca acaagtacag
     3121 atgcaagttc aacctcaaca gtcgaatgca ggagttggtc agcctgcctc tggtgagtcg
     3181 agtctgatta aacagcttct gcttccgaaa cgtggtcctt caacaccagg tggtaagctt
     3241 attctcccag ctccacagat tcctccccct aataatgcaa gagctcctag ccctcaggtg
     3301 gtctatcagg tggccagtaa ccaagccgca ggttttggag tgcaggggca aactccagct
     3361 cagcagctat tggttgggca gcaaaatgtt cagttggtcc caagtgcaat gccaccctca
     3421 gggggagtac aaactgtgcc catttcgaac ttacaaatat tgccaggtcc actgatctca
     3481 aatagcccag caaccatttt ccaagggact tctggcaacc aggtaaccat aacagttgtg
     3541 ccaaatacga gttttgcacc tgcaactgtg agtcagggaa atgcaactca gctcattgct
     3601 ccagcaggaa ttaccatgag cggaacgcag acaggagttg gacttccagt acaaacgctt
     3661 ccagccactc aagcatctcc tgctggacaa tcatcatgta ctactgctac tcccccattc
     3721 aaaggtgata aaataatttg ccaaaaggag gaggaagcaa aggaagcaac aggtttacat
     3781 gttcatgaac gtaaaattga agtcatggag aacccgtcct gccgacgagg agccacaaac
     3841 accagcaatg gggatacaaa ggaaaatgaa atgcatgtgg gaagtctttt aaatgggaga
     3901 aagtacagtg actcaagtct acctccttca aactcaggga aaattcaaag tgagactaat
     3961 cagtgctcac taatcagtaa tgggccatca ttggaattag gtgagaatgg agcatctggg
     4021 aaacagaact cagaacaaat agacatgcaa gatatcaaaa gtgatttgag aaaaccgcta
     4081 gttaatggaa tctgtgattt tgataaagga gatggttctc atttaagcaa aaacattcca
     4141 aatcataaaa cttccaatca tgtaggaaat ggtgagatat ctccaatgga accacaaggg
     4201 actttagata tcactcagca agatactgcc aaaggtgatc aactagaaag aatttctaat
     4261 ggacctgtat taactttggg tggttcatct gtgagcagta tacaggaggc ttcaaatgcg
     4321 gcaacacagc aatttagtgg tactgatttg cttaatggac ctctagcttc aagtttgaat
     4381 tcagatgtgc ctcagcaacg cccaagtgta gttgtctcac cacattctac aacctctgtt
     4441 atacagggac atcaaatcat agcagttccc gactcaggat caaaagtatc ccattctcct
     4501 gccctatcat ctgacgttcg gtctacaaat ggcacagcag aatgcaaaac tgtaaagagg
     4561 ccagcagagg atactgatag ggaaacagtc gcaggaattc caaataaagt aggagttaga
     4621 attgttacaa tcagtgaccc caacaatgct ggctgcagcg caacaatggt tgctgtgcca
     4681 gcaggagcag atccaagcac tgtagctaaa gtagcaatag aaagtgctgt tcagcaaaag
     4741 caacagcatc caccaacata tgtacagaat gtggtcccgc agaacactcc tatgccacct
     4801 tcaccagctg tacaagtgca gggccagcct aacagttctc agccttctcc attcagtgga
     4861 tccagtcagc ctggagatcc aatgagaaaa cctggacaga acttcatgtg tctgtggcag
     4921 tcttgtaaaa agtggtttca gacaccctca caggttttct accatgcagc aactgaacat
     4981 ggaggaaaag atgtatatcc agggcagtgt ctttgggaag gttgtgagcc ttttcagcga
     5041 cagcggtttt cttttattac ccacttgcag gataagcact gttcaaagga tgccctactt
     5101 gcaggattaa aacaagatga accaggacaa gcaggaagtc agaagtcttc taccaagcag
     5161 ccaactgtag ggggcacaag ctcaactcct agagcacaaa aggccattgt gaatcatccc
     5221 agtgctgcac ttatggctct gaggagagga tcaagaaacc ttgtctttcg agattttaca
     5281 gatgaaaaag agggaccaat aactaaacac atccgactaa cagctgcctt aatattaaaa
     5341 aatattggta aatattcaga atgtggtcgc agattgttaa agagacatga aaataactta
     5401 tcagtgctag ccattagtaa catggaagct tcctccaccc ttgccaaatg cctttatgaa
     5461 cttaatttta cagttcagag taaggaacaa gaaaaagact cagaaatgct gcagtagtaa
     5521 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     5581 gacgacttaa gtccattaaa tatgggtgg
//
