LOCUS       sequence-279905-        1415 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1415)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0293
FEATURES             Location/Qualifiers
     source          1..1415
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggggcggaa gaaaatacaa atcacacgca taatggatga aaggaaccga
       61 cagactttaa gaaagaaagg ccttaatggt tgtgagagcc ctgatgctga cgattacttt
      121 gagcacagtc cactctcgga ggacagattc agcaaactaa atgaagatag tgattttatt
      181 ttcaaacgag gccctcctgg tctgccacct cagaactttt caatgtctgt cacagttcca
      241 gtgaccagcc ccaatgcttt gtcctacact aacccaggga gttcactggt gtccccatct
      301 ttggcagcca gctcaacgtt aacagattca agcatgctct ctccacctca aaccacatta
      361 catagaaatg tgtctcctgg agctcctcag agaccaccaa gtactggcaa tgcaggtggg
      421 atgttgagca ctacagacct cacagtgcca aatggagctg gaagcagtcc agtggggaat
      481 ggatttgtaa actcaagagc ttctccaaat ttgattggag ctactggtgc aaatagctta
      541 ggcaaagtca tgcctacaaa gtctccccct ccaccaggtg gtggtaatct tggaatgaac
      601 agtaggaaac cagatcttcg agttgtcatc cccccttcaa gcaagggcat gatgcctcca
      661 ctatcggagg aagaggaatt ggagttgaat acccagagga tcagtagttc tcaagccact
      721 caacctcttg ctaccccagt cgtgtctgtg acaaccccaa gcttgcctcc gcaaggactt
      781 gtgtactcag caatgccgac tgcctacaac actgattatt cactgaccag cgctgacctg
      841 tcagcccttc aaggcttcaa ctcgccagga atgctgtcgc tgggacaggt gtcggcctgg
      901 cagcagcacc acctaggaca agcagccctc agctctcttg ttgctggagg gcagttatct
      961 cagggttcca atttatccat taataccaac caaaacatca gcatcaagtc cgaaccgatt
     1021 tcacctcctc gggatcgtat gaccccatcg ggcttccagc agcagcagca gcagcagcag
     1081 cagcagcagc cgccgccacc accgcagccc cagccacaac ccccgcagcc ccagccccga
     1141 caggaaatgg ggcgctcccc tgtggacagt ctgagcagct ctagtagctc ctatgatggc
     1201 agtgatcggg aggatccacg gggcgacttc cattctccaa ttgtgcttgg ccgaccccca
     1261 aacactgagg acagagaaag cccttctgta aagcgaatga ggatggacgc gtgggtgacc
     1321 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1381 ggaaaggacg agaatctcgg caacggcatg ggtta
//
