LOCUS       sequence-279911-        1619 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0295
FEATURES             Location/Qualifiers
     source          1..1619
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggggcggaa gaaaatacaa atcacacgca taatggatga aaggaaccga
       61 caggtcactt ttacaaagag aaagtttgga ttaatgaaga aagcctatga acttagtgtg
      121 ctctgtgact gtgaaatagc actcatcatt ttcaacagct ctaacaaact gtttcaatat
      181 gctagcactg atatggacaa agttcttctc aagtatacag aatataatga acctcatgaa
      241 agcagaacca actcggatat tgttgagact ttaagaaaga aaggccttaa tggttgtgag
      301 agccctgatg ctgacgatta ctttgagcac agtccactct cggaggacag attcagcaaa
      361 ctaaatgaag atagtgattt tattttcaaa cgaggccctc ctggtctgcc acctcagaac
      421 ttttcaatgt ctgtcacagt tccagtgacc agccccaatg ctttgtccta cactaaccca
      481 gggagttcac tggtgtcccc atctttggca gccagctcaa cgttaacaga ttcaagcatg
      541 ctctctccac ctcaaaccac attacataga aatgtgtctc ctggagctcc tcagagacca
      601 ccaagtactg gcaatgcagg tgggatgttg agcactacag acctcacagt gccaaatgga
      661 gctggaagca gtccagtggg gaatggattt gtaaactcaa gagcttctcc aaatttgatt
      721 ggagctactg gtgcaaatag cttaggcaaa gtcatgccta caaagtctcc ccctccacca
      781 ggtggtggta atcttggaat gaacagtagg aaaccagatc ttcgagttgt catcccccct
      841 tcaagcaagg gcatgatgcc tccactatcg gaggaagagg aattggagtt gaatacccag
      901 aggatcagta gttctcaagc cactcaacct cttgctaccc cagtcgtgtc tgtgacaacc
      961 ccaagcttgc ctccgcaagg acttgtgtac tcagcaatgc cgactgccta caacactgat
     1021 tattcactga ccagcgctga cctgtcagcc cttcaaggct tcaactcgcc aggaatgctg
     1081 tcgctgggac aggtgtcggc ctggcagcag caccacctag gacaagcagc cctcagctct
     1141 cttgttgctg gagggcagtt atctcagggt tccaatttat ccattaatac caaccaaaac
     1201 atcagcatca agtccgaacc gatttcacct cctcgggatc gtatgacccc atcgggcttc
     1261 cagcagcagc agcagcagca gcagcagcag cagccgccgc caccaccgca gccccagcca
     1321 caacccccgc agccccagcc ccgacaggaa atggggcgct cccctgtgga cagtctgagc
     1381 agctctagta gctcctatga tggcagtgat cgggaggatc cacggggcga cttccattct
     1441 ccaattgtgc ttggccgacc cccaaacact gaggacagag aaagcccttc tgtaaagcga
     1501 atgaggatgg acgcgtgggt gacctagtaa tgaactagtc cgtaacttga aagtatttcg
     1561 atttcttggc tttatatatc ttgtggaaag gacgagtgct ctcggtgcgg aggatgtaa
//
