LOCUS       sequence-279914-        1391 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0296
FEATURES             Location/Qualifiers
     source          1..1391
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggggcggaa gaaaatacaa atcacacgca taatggatga aaggaaccga
       61 cagactttaa gaaagaaagg ccttaatggt tgtgagagcc ctgatgctga cgattacttt
      121 gagcacagtc cactctcgga ggacagattc agcaaactaa atgaagatag tgattttatt
      181 ttcaaacgag gccctcctgg tctgccacct cagaactttt caatgtctgt cacagttcca
      241 gtgaccagcc ccaatgcttt gtcctacact aacccaggga gttcactggt gtccccatct
      301 ttggcagcca gctcaacgtt aacagattca agcatgctct ctccacctca aaccacatta
      361 catagaaatg tgtctcctgg agctcctcag agaccaccaa gtactggcaa tgcaggtggg
      421 atgttgagca ctacagacct cacagtgcca aatggagctg gaagcagtcc agtggggaat
      481 ggatttgtaa actcaagagc ttctccaaat ttgattggag ctactggtgc aaatagctta
      541 ggcaaagtca tgcctacaaa gtctccccct ccaccaggtg gtggtaatct tggaatgaac
      601 agtaggaaac cagatcttcg agttgtcatc cccccttcaa gcaagggcat gatgcctcca
      661 ctaaataccc agaggatcag tagttctcaa gccactcaac ctcttgctac cccagtcgtg
      721 tctgtgacaa ccccaagctt gcctccgcaa ggacttgtgt actcagcaat gccgactgcc
      781 tacaacactg attattcact gaccagcgct gacctgtcag cccttcaagg cttcaactcg
      841 ccaggaatgc tgtcgctggg acaggtgtcg gcctggcagc agcaccacct aggacaagca
      901 gccctcagct ctcttgttgc tggagggcag ttatctcagg gttccaattt atccattaat
      961 accaaccaaa acatcagcat caagtccgaa ccgatttcac ctcctcggga tcgtatgacc
     1021 ccatcgggct tccagcagca gcagcagcag cagcagcagc agcagccgcc gccaccaccg
     1081 cagccccagc cacaaccccc gcagccccag ccccgacagg aaatggggcg ctcccctgtg
     1141 gacagtctga gcagctctag tagctcctat gatggcagtg atcgggagga tccacggggc
     1201 gacttccatt ctccaattgt gcttggccga cccccaaaca ctgaggacag agaaagccct
     1261 tctgtaaagc gaatgaggat ggacgcgtgg gtgacctagt aatgaactag tccgtaactt
     1321 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgatat actaaccacg
     1381 cccgttacaa g
//
