LOCUS       sequence-27992-e         642 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Sacristan C, Tussie-Luna MI, Logan SM, Roy AL
  TITLE     Mechanism of Bruton's tyrosine kinase-mediated recruitment and 
            regulation of TFII-I.
  JOURNAL   J Biol Chem. 2004 Feb 20. 279(8):7147-58.
  PUBMED    14623887
REFERENCE   2  (bases 1 to 642)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2004 Feb 20. 279(8):7147-58."
COMMENT     Sequence Label: pEBG-TFII-I L30&37P
FEATURES             Location/Qualifiers
     source          1..642
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..41
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             51..68
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 gtggnnccac catggnnagc agccatcatc atcatcatca cagcagcggc ctggtgccgc
       61 gcggcagcca tatggcccaa gttgcaatgt ccaccctccc cgttgaagat gaggagtcct
      121 cggagagcag gatggtggtg acattcctca tgtcagctcc cgagtccatg tgtaaagaac
      181 cggccaagtc caaagccgaa gtggcctgca ttgcagtgta tgaaacagac gtgtttgtcg
      241 tcggaactga aagaggacgt gcttttgtca ataccagaaa ggattttcaa aaagattttg
      301 taaaatattg tgttgaagaa gaagaaaaag ctgcagagat gcataaaatg aaatctacaa
      361 cccaggcaaa tcggatgagt gtagatgctg tagaaattga aacactcaga aaaacagttg
      421 aggactattt ctgcttttgc tatgggaaag ctttaggcaa atccacagtg gtacctgtac
      481 catatgagaa gatgctgcga gaccagtcgg ctgtggtagt gcaggggctt ccggaaggtg
      541 ttgcctttaa acaccccgag aactatgatc ttgcaaccct gaaatggatt ttggagaaca
      601 aagcaggaat ttcattcatc attaagagac cttttttaga gc
//
