LOCUS       sequence-279923-        1643 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0317
FEATURES             Location/Qualifiers
     source          1..1643
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggggaggaa aaagattcag atccagcgaa tcaccgacga gcggaaccga
       61 caggtgactt tcaccaagcg gaagtttggc ctgatgaaga aggcgtatga gctgagcgtg
      121 ctatgtgact gcgagatcgc actcatcatc ttcaaccact ccaacaagct gttccagtac
      181 gccagcaccg acatggacaa ggtgctgctc aagtacacgg agtacaatga gccacacgag
      241 agccgcacca acgccgacat catcgaggcg ctgcacaaga agcacaggga atgtgagagc
      301 cctgaggtgg acgaggtgtt tgccctgacc ccccagacgg aagagaaata taaaaagata
      361 gacgaggagt ttgataaaat gatgcagagt tatagactgg cctcaactgt cccggccccc
      421 aactttgcca tgcctgtcac ggtgcccgtg tccaatcaga gctcactgca gttcagcaat
      481 cccagcggct ccctggtcac cccttccctg gtgacatcat ccctcacgga cccgcggctc
      541 ctgtcccccc agcagccagc actacagagg aacagtgtgt ctcctggcct gccccagcgg
      601 ccagctagtg cgggggccat gctggggggt gacctgaaca gtgctaacgg agcctgcccc
      661 agccctgttg ggaatggcta cgtcagtgct cgggcttccc ctggcctcct ccctgtggcc
      721 aatggcaaca gcctaaacaa ggtcatccct gccaagtctc cacccccacc tacccacagc
      781 acccagcttg gagcccccag ccgcaagccc gacctgcgag tcatcacttc ccaggcagga
      841 aaggggttaa tgcatcactt gaacaatgcc cagcgccttg gggtctccca gtctactcat
      901 tcgctcacca ccccagtggt ttctgtggca acgccgagtt tactcagcca gggcctcccc
      961 ttctcttcca tgcccactgc ctacaacaca gattaccagt tgaccagtgc agagctctcc
     1021 tccttaccag cctttagttc acctgggggg ctgtcgctag gcaatgtcac tgcctggcaa
     1081 cagccacagc agccccagca gccgcagcag ccacagcctc cacagcagca gccaccgcag
     1141 ccacagcagc cacagccaca gcagcctcag cagccgcaac agccacctca gcaacagtcc
     1201 cacctggtcc ctgtatctct cagcaacctc atcccgggca gccccctgcc ccacgtgggt
     1261 gctgccctca cagtcaccac ccacccccac atcagcatca agtcagaacc ggtgtcccca
     1321 agccgtgagc gcagccctgc gcctccccct ccagctgtgt tcccagctgc ccgccctgag
     1381 cctggcgatg gtctcagcag cccagccggg ggatcctatg agacgggaga ccgggatgac
     1441 ggacgggggg acttcgggcc cacactgggc ctgctgcgcc cagccccaga gcctgaggct
     1501 gagggctcag ctgtgaagag gatgcggctt gatacctgga cattaaagta gtaatgaact
     1561 agtccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgag
     1621 actgcagtag ccgcagcggt acg
//
