LOCUS       sequence-279926-        1058 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0324
FEATURES             Location/Qualifiers
     source          1..1058
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacaaggg aagagagaga atggccgctg ccgccgccgc tgctgccgcc
       61 gccgccgccg ccgctcagtg ccggagccct cggtgcgcgg cggagagaag aggattccgg
      121 cgggaactcg actcttggcg ccaccgcctc atgcactgtg taggttttga gagtatttta
      181 gaagggcttt atggaccacg gctacgaaga gacctcagtt tatttgaaga ctgtgaacca
      241 gaagagctga ctgactggtc tatggatgaa aaatgttcat tttgtaacct acagagagaa
      301 gcagtcagtg attgtatacc atctcttgat tcttcacagt caacaccaac agaggagcta
      361 tcatctcagg gccagtccaa cactgataag attgaatgcc aagcagaaaa ttacctaaat
      421 gcactctttc gaaagaaaga tcttcctcag aactgtgatc ctaacattcc cctagttgct
      481 caggaattaa tgaaaaagat gatacgtcaa tttgcgattg agtacatttc aaaaagtggt
      541 aaaactcaag agaatagaaa tggttcaatt ggaccaagta tagtatgtaa aagtatccaa
      601 atgaatcaag cagaaaactc ccttcaggaa gagcaggaag gccccttaga cctcactgtg
      661 aatcgaatgc aagaacaaaa tactcagcaa ggggatggag tgttagatct ctctacaaag
      721 aaaaccagca taaaatctga agagtcatcc atatgtgatc cttcttctga aaattcagtg
      781 gctggcatgc tacaagtgaa aactgatgag aaactgaatg tgtctgatga gaatacagca
      841 agttgccctt tgagccccat taagatgtgc cttaatcgtc ccattgaatg gaatctgaat
      901 ttgacaacag catctctaac gagctgtaca gttcataacc aaaatctcaa aagcgaagag
      961 aaatagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1021 tgtggaaagg acgacacctt agcacccgct ctcaacca
//
