LOCUS       sequence-279929-        1910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0325
FEATURES             Location/Qualifiers
     source          1..1910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacaaggg aagagagaga atggccgctg ccgccgccgc tgctgccgcc
       61 gccgccgccg ccgctcagtg ccggagccct cggtgcgcgg cggagagaag aggattccgg
      121 cgggaactcg actcttggcg ccaccgcctc atgcactgtg taggttttga gagtatttta
      181 gaagggcttt atggaccacg gctacgaaga gacctcagtt tatttgaaga ctgtgaacca
      241 gaagagctga ctgactggtc tatggatgaa aaatgttcat tttgtaacct acagagagaa
      301 gcagtcagtg attgtatacc atctcttgat tcttcacagt caacaccaac agaggagcta
      361 tcatctcagg gccagtccaa cactgataag attgaatgcc aagcagaaaa ttacctaaat
      421 gcactctttc gaaagaaaga tcttcctcag aactgtgatc ctaacattcc cctagttgct
      481 caggaattaa tgaaaaagat gatacgtcaa tttgcgattg agtacatttc aaaaagtggt
      541 aaaactcaag agaatagaaa tggttcaatt ggaccaagta tagtatgtaa aagtatccaa
      601 atgaatcaag cagaaaactc ccttcaggaa gagcaggaag gccccttaga cctcactgtg
      661 aatcgaatgc aagaacaaaa tactcagcaa ggggatggag tgttagatct ctctacaaag
      721 aaaaccagca taaaatctga agagtcatcc atatgtgatc cttcttctga aaattcagtg
      781 gctgggagac tacacagaaa cagagaggac tatgtggaaa gaagtgctga gtttgcagat
      841 ggtttgctct caaaagcttt gaaagacatt cagtctggag cactggacat aaataaagca
      901 ggcatacttt atggcatacc tcaaaaaact ttacttcttc acttagaagc cttaccagca
      961 gggaagcctg catcttttaa aaacaaaact cgagatttcc atgatagtta ttcatataag
     1021 gacagtaaag aaacttgtgc agtgctgcaa aaagtagcct tgtgggcaag agctcaagca
     1081 gagcgcacag aaaaaagtaa actcaatcta cttgaaacct cagaaataaa attcccaaca
     1141 gcttccactt acctccatca gctaactcta cagaaaatgg tcactcagtt taaagaaaaa
     1201 aatgaaagcc tccaatatga aacttcaaat cctactgtac agttaaaaat tcctcagcta
     1261 cgagtaagtt ctgtctcaaa atcacaacct gatggttctg gtctgttgga tgttatgtat
     1321 caagtttcca aaacctcttc agtcctagaa ggatcagctc tccaaaaact gaaaaatata
     1381 ctccctaaac agaacaaaat agaatgttct gggcctgtaa ctcactcaag tgttgactct
     1441 tactttctac atggggacct ctctcctttg tgtcttaatt ctaaaaatgg aacagttgat
     1501 ggaacctctg aaaatactga agatggatta gatcgaaaag acagtaagca gcccaggaaa
     1561 aaacgtggcc gctatcggca atatgatcat gaaataatgg aagaagctat tgcaatggta
     1621 atgagcggaa aaatgagtgt ttccaaagca caaggaattt atggggtacc tcacagcact
     1681 ttagaataca aggtaaaaga aagatctgga acactgaaga ctcctccgaa gaagaaacta
     1741 cgattaccag acactgggtt atataatatg acagattcag ggactggcag ctgcaaaaac
     1801 agcagcaagc ctgtgtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     1861 ctttatatat cttgtggaaa ggacgaaggc gcacgattaa gatagtctcg
//
