LOCUS       sequence-279932-        2357 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0326
FEATURES             Location/Qualifiers
     source          1..2357
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggacctca gggagccagg agacaggctt tcttggcatt tggggatgtc
       61 actgtggatt tcacccagaa ggaatggagg ctgctgagcc ctgctcagag ggccctgtac
      121 agggaggtga cactggagaa ctacagccac ctggtctcac taggaattct ccattctaaa
      181 ccagaactca tcaggcggct agagcaaggg gaagtgccct ggggagaaga gagaagacgc
      241 cggccaggcc cctgtgcagg aatatatgca gaacatgtcc tgcggcccaa gaatcttgga
      301 cttgcacatc agaggcaaca gcaactacaa ttttctgatc aaagcttcca gagtgacaca
      361 gctgaaggtc aagagaaaga aaaaagcact aagcccatgg cattttccag cccaccccta
      421 agacatgcag taagctcaag gaggaggaac agtgtagtgg aaatagagtc tagtcaaggc
      481 cagagggaaa atcctacaga aatagacaaa gtattgaaag gaatagaaaa ttcaagatgg
      541 ggagcattca agtgtgcaga gcgtgggcaa gacttcagcc ggaagatgat ggtaatcata
      601 cacaaaaaag cacattccag gcagaaactt tttacatgca gggagtgtca ccagggcttt
      661 agagatgagt cagcattgct cttgcaccag aacacacaca caggagagaa gtcctatgtg
      721 tgcagtgtgt gtgggcgagg cttcagcctc aaggccaacc tcctcagaca ccagaggaca
      781 cactcaggag agaagccttt tctgtgcaag gtgtgtggac gaggctatac cagtaagtca
      841 tacctcactg tgcatgagag aacacacaca ggagagaagc cttatgaatg ccaggagtgt
      901 gggcgaaggt ttaacgataa gtcctcatac aacaagcact tgaaggcgca ttcaggggag
      961 aagccttttg tgtgcaagga gtgtgggcga ggctatacta ataagtcata cttcgttgtg
     1021 cacaagagaa tacactcagg agagaagcct tacagatgcc aggagtgtgg ccgaggcttt
     1081 agcaataagt cacaccttat cacacaccag aggacacact caggggagaa gccctttgcg
     1141 tgcaggcagt gtaagcaaag ttttagcgtg aaaggaagtc tcctcagaca ccagagaaca
     1201 cactcagggg agaagccttt tgtgtgcaag gattgtgagc gaagctttag ccaaaagtca
     1261 actcttgtct accaccagag aacacactca ggggagaaac cttttgtttg tagagaatgt
     1321 gggcaaggat ttattcagaa gtcaaccctt gtgaaacatc agatcacaca ctcagaggag
     1381 aagccttttg tgtgcaagga ctgtggacga ggctttatcc aaaagtcaac cttcacttta
     1441 caccagagga cacactcaga ggagaagcct tatggatgtc gggagtgtgg gcgaaggttt
     1501 cgggataagt cctcctataa caagcacctg agggcacact tgggtgagaa acgttttttc
     1561 tgcagggatt gtgggcgagg ctttaccttg aagccaaatc tcaccataca tcagaggaca
     1621 cactcaggag agaagccctt catgtgcaag cagtgtgaga aaagttttag tttgaaggca
     1681 aatcttctta gacatcagtg gacacactcg ggggaaaggc catttaattg caaggattgc
     1741 gggcgaggct tcatcctaaa atcaactctc ctcttccacc agaagacaca ctcaggggag
     1801 aagcctttca tctgtagtga atgtgggcaa ggatttatct ggaagtcaaa tcttgtgaaa
     1861 caccagcttg cacattctgg caagcagcct tttgtatgca aggagtgtgg gcgaggcttc
     1921 aactggaagg gaaatctcct cacacaccag aggacacact caggggagaa gcccttcgtg
     1981 tgtaatgtgt gtgggcaagg cttcagctgg aagagaagtc tcaccagaca ccactggcgg
     2041 atacactcaa aggagaagcc ttttgtttgc caggagtgta agcgaggcta taccagtaag
     2101 tcagacctca ctgtgcatga aagaatacac acaggagaga ggccttatga atgccaagag
     2161 tgtggacgaa agtttagcaa taagtcatac tacagtaagc acttaaagag acacttacgt
     2221 gagaagcgtt tttgtacagg gagtgtgggt gaggcttcat cttagtaatg aactagtccg
     2281 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaaagttc
     2341 acgaagtccg cagccgt
//
