LOCUS       sequence-279938-        2099 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0333
FEATURES             Location/Qualifiers
     source          1..2099
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaatccgt cacctttgag gatgtggccg tggagttcat ccaggagtgg
       61 gcattgctgg acagcgcacg gaggagcctg tgcaaataca ggatgcttga ccagtgcagg
      121 accctggcct ccaggggaac tccaccatgc aaacccagtt gtgtctccca gctggggcaa
      181 agagcagagc caaaggcaac agaacgaggg attctccgtg ccacaggtgt tgcctgggaa
      241 tctcaactta aacccgaaga gttgccttct atgcaggatc ttttggaaga agcatcctcc
      301 agggacatgc aaatggggcc ggggctgttc ctgaggatgc agctggtgcc ctccatagaa
      361 gagagggaga caccattgac tcgagaggac cggccagctc tccaggagcc gccttggtct
      421 ctgggatgca cgggactgaa ggccgctatg cagattcaga gggtggtgat accagtgcct
      481 actctgggcc accgcaaccc atgggtggcc agggattctg ctgtgcctgc acgtgaccct
      541 gcctggcttc aggaggacaa agtggaggaa gaagctatgg ctcctgggct gccaaccgcc
      601 tgttcacagg aaccagtcac ctttgcagat gtggctgtgg tgttcacccc agaagaatgg
      661 gtgtttctgg actctactca gaggagcctg tatagagatg tgatgctgga gaactacagg
      721 aacctggcct ctgtggctga tcaactgtgc aaacccaatg cgttgtctta tttggaagaa
      781 agaggagagc agtggaccac tgacaggggc gtcctctcag acacctgtgc agaacctcag
      841 tgtcaacccc aagaggcaat tcctagccaa gatactttta cagagatcct gtccattgat
      901 gtgaaagggg agcaacctca gcctggagaa aaactctata aatataatga acttgagaaa
      961 ccttttaaca gcattgaacc acttttccag taccagagaa ttcatgctgg agaggcatcc
     1021 tgtgaatgtc aagagattag aaattccttc ttccagagtg cccacctaat tgtgcccgag
     1081 aaaatccgta gtggggataa atcctatgca tgtaacaaat gtgaaaaatc cttcagatac
     1141 agctctgacc ttatcaggca tgagaagact catactgcag agaagtgctt tgactgtcaa
     1201 gaatgtgggc aagccttcaa atattcctcg aatctccggc gacacatgag aacccatacc
     1261 ggagagaagc catttgaatg tagtcagtgt gggaaaacct tcacgaggaa ctttaacctg
     1321 attttgcacc agagaaacca cacaggagag aagccctacg agtgtaaaga ttgtgggaaa
     1381 gccttcaatc agccatcatc cctcaggagc cacgtgagaa ctcacactgg agagaagccc
     1441 tttgaatgca gccagtgtgg gaaagccttc agggaacact cttcactgaa gacacatctg
     1501 cgaacccata ccagagagaa accatatgaa tgcaaccagt gtggcaagcc cttccggacg
     1561 agcactcatc tgaacgtgca caagaggata cacacagggg agaaactgta tgagtgcgcg
     1621 acttgcggtc aggtcttgag tcgtctttca accctgaaga gtcacatgcg aactcacact
     1681 ggagagaagc cctatgtgtg ccaggaatgt gggcgagcct tcagtgagcc ctcatccctc
     1741 aggaaacatg caaggactca cagtggcaag aagccctatg catgccagga atgcgggcga
     1801 gcctttggtc agtcttcaca tcttattgta catgtgagaa cacacagtgc cgggagaccc
     1861 tatcaatgta atcagtgtga gaaagccttc aggcacagct cctcactcac tgtacacaaa
     1921 agaacccatg tgggaagaga gaccattagg aatggcagcc tgcctttatc catgtctcat
     1981 ccatactgtg ggccccttgc taattagtaa tgaactagtc cgtaacttga aagtatttcg
     2041 atttcttggc tttatatatc ttgtggaaag gacgagcttc ttcatcccgt ttcaccaca
//
