LOCUS       sequence-279950-         458 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0363
FEATURES             Location/Qualifiers
     source          1..458
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacgtgtt cctcatgatc cggcgccaca agaccaccat cttcacggac
       61 gccaaggagt ccagcacggt gttcgaactg aagcgcatcg tcgagggcat cctcaagcgg
      121 cctcctgacg agcagcggct gtacaaggat gaccaactct tggatgatgg caagacactg
      181 ggcgagtgtg gcttcaccag tcaaacagca cggccacagg ccccagccac agtggggctg
      241 gccttccggg cagatgacac ctttgaggcc ctgtgcatcg agccgttttc cagcccgcca
      301 gagctgcccg atgtgatgaa gccccaggac tcgggaagca gtgccaatga acaagccgtg
      361 cagtagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
      421 tgtggaaagg acgagtccat ggtccatacg tgacgcat
//
