LOCUS       sequence-279974-        2195 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0393
FEATURES             Location/Qualifiers
     source          1..2195
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggattcct ggggtgctgg tgtgtgtcct ttcaggaggt ggtaacattt
       61 ggcgatgtgg ctgtgcactt ctctcgggag gagtggcagt gtctggaccc tggccagagg
      121 gccctctaca gggaagtgat gctggagaac cacagcagtg tggctggact agcaggattc
      181 ctggttttca agcctgagct gatctctcgg ctggagcagg gagaagagcc atgggtcctc
      241 gacctgcagg gagcagaggg gacagaggca ccaaggacct ccaagacaga ttctacgatt
      301 aggactgaaa atgagcaggc ctgtgaggac atggacatcc taaaatcaga atcctatggg
      361 acagtggtca gaatctcccc acaggacttt cctcagaatc ctggctttgg agacgtttct
      421 gattctgagg tctggttaga cagtcatctg ggcagtcccg ggctgaaagt gacaggcttt
      481 accttccaaa ataactgttt gaatgaggag actgtggttc ccaagacctt caccaaggac
      541 gcaccccagg gatgtaagga gctgggaagc agcggcctgg attgtcagcc tcttgaaagt
      601 cagggagaga gtgcggaagg gatgtcccag agatgcgagg agtgtggcaa aggcatcaga
      661 gccacttcag atatcgctct gcattgggaa attaatacac agaaaattag cagatgtcaa
      721 gaatgccaaa aaaagttatc tgactgcttg caggggaaac atacaaataa ctgccatgga
      781 gagaagccgt acgaatgtgc agagtgtggg aaagtcttca ggctctgctc gcagcttaat
      841 cagcatcaga gaatccacac gggagagaaa ccctttaaat gcactgagtg tggaaaagcc
      901 ttccgcctga gctcaaaact tattcagcat caaagaatcc acactgggga gaagccctac
      961 agatgtgagg aatgtggaaa agcttttggt cagagctcaa gcctcatcca ccatcagaga
     1021 atccacacag gagagaggcc ctatggttgt cgtgagtgtg ggaaagcctt cagccagcag
     1081 tcgcagctgg ttagacacca gagaactcac actggggaga ggccctaccc ttgcaaggag
     1141 tgtgggaagg ccttcagcca gagctccacc ctagcccagc atcaaaggat gcatactggg
     1201 gagaaagctc aaattctaaa agcctcagac agtccaagcc ttgttgcaca tcagagaatt
     1261 cacgctgtag agaaaccatt taagtgtgat gagtgtggga aagcttttag gtggatctct
     1321 cgcctgagtc agcatcagct gattcacact ggagagaagc cttataaatg caacaagtgt
     1381 acaaaagcct ttggttgtag ttcacggctt attcgccatc agagaactca cactggagaa
     1441 aaaccattta aatgtgatga gtgtggcaaa ggctttgttc agggctcaca ccttattcag
     1501 catcagcgaa tccacactgg agagaaaccc tatgtgtgta atgactgtgg aaaagccttc
     1561 agtcagagtt ccagccttat ttaccatcag agaatccata aaggagagaa gccctacgaa
     1621 tgcctccaat gcggaaaagc cttcagtatg agcacacagc ttacaataca tcaaagggtt
     1681 cacactggag agaggcccta taaatgtaat gaatgtggga aagccttcag tcaaaactca
     1741 acccttttcc aacaccagat aattcatgca ggggtgaagc cctatgagtg cagtgagtgt
     1801 ggaaaagcct tcagccggag ctcatatctt attgaacacc agagaataca cactagggcc
     1861 cagtggtttt acgaatatgg gaatgccctg gaagggtcca cctttgtgag ccgtaaaaag
     1921 gttaatacta taaagaaact gcatcagtgt gaagactgtg agaagatatt taggtggcgt
     1981 tcacacctaa ttatacacca gagaattcac accggggaga agccttataa atgcaatgac
     2041 tgtggcaaag cttttaatcg tagctcaagg cttacccagc atcaaaaaat tcacatggga
     2101 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     2161 ggaaaggacg atgaacaagt actccgttag gagag
//
