LOCUS       sequence-27998-e         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Cheriyath V, Roy AL
  TITLE     Structure-function analysis of TFII-I. Roles of the N-terminal end, 
            basic region, and I-repeats.
  JOURNAL   J Biol Chem. 2001 Mar 16. 276(11):8377-83.
  PUBMED    11113127
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2001 Mar 16. 276(11):8377-83."
COMMENT     Sequence Label: pEBG-TFII-I deltaBR
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..18
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             34..51
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             61..78
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ctggttccgc gtggatccac catgggcagc agccatcatc atcatcatca cagcagcggc
       61 ctggtgccgc gcggcagcca tatggcccaa gttgcaatgt ccaccctccc cgttgaagat
      121 gaggagtcct cggagagcag gatggtggtg acattcctca tgtcagctct cgagtccatg
      181 tgtaaagaac tggccaagtc caaagccgaa gtggcctgca ttgcagtgta tgaaacagac
      241 gtgtttgtcg tcggaactga aagaggacgt gcttttgtca ataccagaaa ggattttcaa
      301 aaagattttg taaaatattg tgttgaagaa gaagaaaaag ctgcagagat gcataaaatg
      361 aaatctacaa cccaggcaaa tcggatgagt gtagatgctg tagaaattga aacactcaga
      421 aaaacagttg aggactattt ctgcttttgc tatgggaaag ctttaggcaa atccacagtg
      481 gtacctgtac catatgagaa gatgctgcga gaccagtcgg ctgtggtagt gcaggggctt
      541 ccggaaggtg ttgcctttaa acaccccgag aactatgatc ttgcaaccct gaaatggatt
      601 ttggagaaca aagcaggaat ttcattcatc attaagagac cttttttaga gccaaagaag
      661 catgt
//
