LOCUS       sequence-279980-        1265 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1265)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0400
FEATURES             Location/Qualifiers
     source          1..1265
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgctgt gtctccgacc accagatgcc aggaatcagt gacattcgaa
       61 gacgttgccg tggttttcac agatgaagag tggagtcgtc tggtccccat acagagggac
      121 ctctacaagg aggtgatgct ggagaactat aacagcattg tgtcattgga ctgggaaact
      181 aagcctgaga ttcacgatgc ttcagacaaa aaatcagaag gatcattgag ggaatgcctt
      241 ggaaggcaaa gtcctctgtg tcctaaattt gaagttcata cacccaatgg caggatggga
      301 acagaaaagc aaagcccttc aggggagact cgtaagaaat ccctctcccg ggacaaaggc
      361 ttgcggcgac ggtcagccct gtccagggaa attctcacta aagagagaca ccaggaatgc
      421 agtgactgtg ggaagacctt ttttgaccac tcatccctca cccgccatca gaggactcac
      481 actggggaga agccctacga ctgccgcgag tgtgggaaag ccttcagcca caggagcagc
      541 ctcagcagac atctgatgtc acacactggg gagagcccct acgagtgcag tgtgtgctca
      601 aaagccttct ttgaccgttc gtccctaact gtccatcagc gaattcacac tggagagaaa
      661 ccctttcagt gcaacgagtg tggaaaagcc ttttttgacc gttcatccct tactcgacac
      721 cagagaattc acactggaga aagtccttat gaatgtcatc agtgtgggaa agcctttagc
      781 cagaaaagta ttcttactcg ccatcagcta atccacactg gcaggaagcc ttatgagtgt
      841 aacgagtgcg ggaaagcttt ctatggtgtc tcgtctctga atagacatca gaaagctcat
      901 gctggggacc ctcgctatca gtgtaacgag tgtggcaaag ctttctttga ccgctcatcc
      961 cttacacagc atcagaagat ccacactgga gacaagccat atgaatgcag cgaatgcggg
     1021 aaagccttta gccagcggtg ccggctcacg cggcatcagc gtgtccacac gggagagaag
     1081 ccctttgaat gcactgtgtg tgggaaagtt ttcagttcaa aatcttctgt tattcaacat
     1141 caacggcgtt acgccaaaca gggaatagac tagtaatgaa ctagtccgta acttgaaagt
     1201 atttcgattt cttggcttta tatatcttgt ggaaaggacg atagtttaac tgcaatagcc
     1261 cgttc
//
