LOCUS       sequence-279983-        1379 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1379)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0401
FEATURES             Location/Qualifiers
     source          1..1379
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgctgt gtctccgacc accagatgcc aggaatcagt gacattcgaa
       61 gacgttgccg tggttttcac agatgaagag tggagtcgtc tggtccccat acagagggac
      121 ctctacaagg aggtgatgct ggagaactat aacagcattg tgtcattggg ccttccagtt
      181 cctcaacctg atgtgatttt ccaattgaag agaggggaca agccgtggat ggtagatctt
      241 catgggtctg aggagagaga atggccagag agtgtctctc tagactggga aactaagcct
      301 gagattcacg atgcttcaga caaaaaatca gaaggatcat tgagggaatg ccttggaagg
      361 caaagtcctc tgtgtcctaa atttgaagtt catacaccca atggcaggat gggaacagaa
      421 aagcaaagcc cttcagggga gactcgtaag aaatccctct cccgggacaa aggcttgcgg
      481 cgacggtcag ccctgtccag ggaaattctc actaaagaga gacaccagga atgcagtgac
      541 tgtgggaaga ccttttttga ccactcatcc ctcacccgcc atcagaggac tcacactggg
      601 gagaagccct acgactgccg cgagtgtggg aaagccttca gccacaggag cagcctcagc
      661 agacatctga tgtcacacac tggggagagc ccctacgagt gcagtgtgtg ctcaaaagcc
      721 ttctttgacc gttcgtccct aactgtccat cagcgaattc acactggaga gaaacccttt
      781 cagtgcaacg agtgtggaaa agcctttttt gaccgttcat cccttactcg acaccagaga
      841 attcacactg gagaaagtcc ttatgaatgt catcagtgtg ggaaagcctt tagccagaaa
      901 agtattctta ctcgccatca gctaatccac actggcagga agccttatga gtgtaacgag
      961 tgcgggaaag ctttctatgg tgtctcgtct ctgaatagac atcagaaagc tcatgctggg
     1021 gaccctcgct atcagtgtaa cgagtgtggc aaagctttct ttgaccgctc atcccttaca
     1081 cagcatcaga agatccacac tggagacaag ccatatgaat gcagcgaatg cgggaaagcc
     1141 tttagccagc ggtgccggct cacgcggcat cagcgtgtcc acacgggaga gaagcccttt
     1201 gaatgcactg tgtgtgggaa agttttcagt tcaaaatctt ctgttattca acatcaacgg
     1261 cgttacgcca aacagggaat agactagtaa tgaactagtc cgtaacttga aagtatttcg
     1321 atttcttggc tttatatatc ttgtggaaag gacgatttcg accatccgag ccgcataag
//
