LOCUS       sequence-279998-        1394 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0427
FEATURES             Location/Qualifiers
     source          1..1394
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacgagca gcccaggctg atgcattccc atgctggggt cgggatggcc
       61 ggacaccccg gcctgtccca gcacttgcag gatggggccg gagggaccga gggggagggc
      121 gggaggaagc aggacattgg agacatttta cagcaaatta tgaccatcac agaccagagt
      181 ttggatgagg cgcaggccag aaaacatgct ttaaactgcc acagaatgaa gcctgccttg
      241 tttaatgtgt tgtgtgaaat caaagaaaaa acagttttga gtatccgagg agcccaggag
      301 gaggaaccca cagaccccca gctgatgcgg ctggacaaca tgctgttagc ggaaggcgtg
      361 gcggggcctg agaagggcgg agggtcggcg gcagcggcgg cagcggcggc ggcttctgga
      421 ggggcaggtt cagacaactc agtggagcat tcagattaca gagccaaact ctcacagatc
      481 agacaaatct accatacgga gctggagaaa tacgagcagg cctgcaacga gttcaccacc
      541 cacgtgatga atctcctgcg agagcaaagc cggaccaggc ccatctcccc aaaggagatt
      601 gagcggatgg tcagcatcat ccaccgcaag ttcagctcca tccagatgca gctcaagcag
      661 agcacgtgcg aggcggtgat gatcctgcgt tcccgatttc tggatgcgcg gcggaagaga
      721 cggaatttca acaagcaagc gacagaaatc ctgaatgaat atttctattc ccatctcagc
      781 aacccttacc ccagtgagga agccaaagag gagttagcca agaagtgtgg catcacagtc
      841 tcccaggtat caaactggtt tggaaataag cgaatccggt acaagaagaa cataggtaaa
      901 tttcaagagg aagccaatat ttatgctgcc aaaacagctg tcactgctac caatgtgtca
      961 gcccatggaa gccaagctaa ctcgccctca actcccaact cggctggttc ttccagttct
     1021 tttaacatgt caaactctgg agatttgttc atgagcgtgc agtcactcaa tggggattct
     1081 taccaagggg cccaggttgg agccaacgtg caatcacagg tggataccct tcgccatgtt
     1141 atcagccaga caggaggata cagtgatgga ctcgcagcca gtcagatgta cagtccgcag
     1201 ggcatcagtg ctaatggagg ttggcaggat gctactaccc cttcatcagt gacctcccct
     1261 acagaaggcc ctggcagtgt tcactctgat acctccaact agtaatgaac tagtccgtaa
     1321 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga atacccgacc
     1381 ggttacaaca cgac
//
