LOCUS       sequence-280004-        1739 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0431
FEATURES             Location/Qualifiers
     source          1..1739
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcgacgga cgcggcgcta cgccggcttc tgaggctgca ccgcacggag
       61 atcgcggtgg ccgtggacag cgccttccca ctgctgcacg cgctggctga ccacgacgtg
      121 gtccccgagg acaagtttca ggagacgctt catctgaagg aaaaggaggg ctgcccccag
      181 gccttccacg ccctcctgtc ctggctgctg acccaggact ccacagccat cctggacttc
      241 tggagggtgc tgttcaagga ctacaacctg gagcgctatg gccggctgca gcccatcctg
      301 gacagcttcc ccaaagatgt ggacctcagc cagccccgga aggggaggaa gcccccggcc
      361 gtccccaagg ctttggtacc gccacccaga ctccccacca agaggaaggc ctcagaagag
      421 gctcgagctg ccgcgccagc agccctgact ccaaggggca ccgccagccc aggctctcaa
      481 ctgaaggcca agccccccaa gaagccggag agcagcgcag agcagcagcg ccttccactc
      541 gggaacggga ttcagaccat gtcagcttca gtccagagag ctgtggccat gtcctccggg
      601 gacgtcccgg gagcccgagg ggccgtggag gggatcctca tccagcaggt gtttgagtca
      661 ggcggctcca agaagtgcat ccaggttggc ggggagttct acactcccag caagttcgaa
      721 gactccggca gtgggaagaa caaggcccgc agcagcagtg gcccgaagcc tctggttcga
      781 gccaagggag cccagggcgc tgcccccggt ggaggtgagg ctaggctggg ccagcagggc
      841 agcgttcccg cccctctggc cctccccagt gacccccagc tccaccagaa gaatgaggac
      901 gagtgtgccg tgtgtcggga cggcggggag ctcatctgct gtgacggctg ccctcgggcc
      961 ttccacctgg cctgcctgtc ccctccgctc cgggagatcc ccagtgggac ctggaggtgc
     1021 tccagctgcc tgcaggcaac agtccaggag gtgcagcccc gggcagagga gccccggccc
     1081 caggagccac ccgtggagac cccgctcccc ccggggctta ggtcggcggg agaggaggta
     1141 agaggtccac ctggggaacc cctagccggc atggacacga ctcttgtcta caagcacctg
     1201 ccggctccgc cttctgcagc cccgctgcca gggctggact cctcggccct gcacccccta
     1261 ctgtgtgtgg gtcctgaggg tcagcagaac ctggctcctg gtgcgcgttg cggggtgtgc
     1321 ggagatggta cggacgtgct gcggtgtact cactgcgccg ctgccttcca ctggcgctgc
     1381 cacttcccag ccggcacctc ccggcccggg acgggcctgc gctgcagatc ctgctcagga
     1441 gacgtgaccc cagcccctgt ggagggggtg ctggccccca gccccgcccg cctggcccct
     1501 gggcctgcca aggatgacac tgccagtcac gagcccgctc tgcacaggga tgacctggag
     1561 tcccttctga gcgagcacac cttcgatggc atcctgcagt gggccatcca gagcatggcc
     1621 cgtccggcgg cccccttccc ctcctagtaa tgaactagtc cgtaacttga aagtatttcg
     1681 atttcttggc tttatatatc ttgtggaaag gacgacacta gggcgaggga gataatagg
//
