LOCUS       sequence-280007-        1328 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1328)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0435
FEATURES             Location/Qualifiers
     source          1..1328
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggaagg actctgggag cagggttttc tgcggacccc agaacagtgt
       61 cgcaccaagt tcaaaagcct acagttgagt taccgcaaag tgaggagagg ccgtgtgcct
      121 gagccttgta tcttttatga ggaaatgaat gctctttcag gctcctgggc ctctgcacct
      181 cctatggcaa gcgatgctgt tcctggccaa gaaggaagtg atattgaggc tggagagctg
      241 aatcaccaga atggggaacc cacggaggta gaagatggca ctgtggatgg tgcagacagg
      301 gatgaaaagg acttcaggaa tcctggccag gaagtcagga aactagacct gccagtgctg
      361 ttcccaaaca gacttggttt tgagttcaag aacgagatta aaaaagaaaa tctaaaatgg
      421 gatgattcag aggaagtaga aataaacaag gctttacaga gaaagtccag aggagtttat
      481 tggcactctg agctacaaaa aggcttggag agtgagccaa catcaagaag gcaatgtaga
      541 aattctccag gggagagtga ggagaaaacc ccatcccagg agaagatgag tcaccagagt
      601 ttttgtgcca gggacaaagc ctgtacacat atcctctgtg ggaaaaactg ctctcagagt
      661 gtgcactctc cccacaagcc agcgctcaaa ctggaaaaag tatctcaatg tcctgaatgt
      721 gggaaaacct ttagccgaag ttcttatctt gttcggcatc aaagaatcca cacaggcgag
      781 aagcctcaca agtgcagtga gtgcgggaag ggctttagtg agcgctccaa cctcactgcc
      841 cacctacgaa ctcacacagg ggagaggccc tatcagtgtg ggcaatgtgg gaaaagcttc
      901 aaccagagtt ccagcctcat tgtccaccag aggacccata ccggggaaaa gccttaccag
      961 tgcattgtct gtggaaagag attcaacaac agttcccagt tcagtgctca ccggcgcatc
     1021 cacactgggg agagcccata caagtgtgca gtgtgtggga aaatcttcaa caatagctcc
     1081 cacttcagtg cccaccgaaa aacccacact ggtgaaaagc cttacaggtg ttctcactgt
     1141 gagagaggct tcactaagaa ctctgccctc acccgtcatc agacagtaca catgaaagca
     1201 gtactctcat cacaggaagg aagagatgcg ttatagtaat gaactagtcc gtaacttgaa
     1261 agtatttcga tttcttggct ttatatatct tgtggaaagg acgatatgca cctgacacac
     1321 gattggtg
//
