LOCUS       sequence-28001-e         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Cheriyath V, Novina CD, Roy AL
  TITLE     TFII-I regulates Vbeta promoter activity through an initiator 
            element.
  JOURNAL   Mol Cell Biol. 1998 Aug . 18(8):4444-54.
  PUBMED    9671454
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 1998 Aug . 18(8):4444-54."
COMMENT     Sequence Label: pEBG-TFII-I-p70
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             18..35
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             45..62
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 ccaccatggg cagcagccat catcatcatc atcacagcag cggcctggtg ccgcgcggca
       61 gccatatggc ccaagttgca atgtccaccc tccccgttga agatgaggag tcctcggaga
      121 gcaggatggt ggtgacattc ctcatgtcag ctctcgagtc catgtgtaaa gaactggcca
      181 agtccaaagc cgaagtggcc tgcattgcag tgtatgaaac agacgtgttt gtcgtcggaa
      241 ctgaaagagg acgtgctttt gtcaatacca gaaaggattt tcaaaaagat tttgtaaaat
      301 attgtgttga agaagaagaa aaagctgcag agatgcataa aatgaaatct acaacccagg
      361 caaatcggat gagtgtagat gctgtagaaa ttgaaacact cagaaaaaca gttgaggact
      421 atttctgctt ttgctatggg aaagctttag gcaaatccac agtggtacct gtaccatatg
      481 agaagatgct gcgagaccag tcggctgtgg tagtgcaggg gcttccggaa ggngttgcct
      541 ttaaacaccc cgagaactat gatcttgcaa ccctgaaatg gattttggag aacaaagcag
      601 gaatttcatt catcattaag agaccttttt ttagagccaa agaagcatgt angtggtcgt
      661 gtgatggtaa cagatgctga caggtcaata ctatctccan gtggaagttg tgg
//
