LOCUS       sequence-280013-        1934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1934)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0440
FEATURES             Location/Qualifiers
     source          1..1934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgaaga tgaatttgaa ttgcagccac aagagccaaa ctcatttttt
       61 gatggaatag tggatgatgc tggcaaaata gaacatgatg gttccactgg agtgaccatc
      121 gatgcagaat cagaaatgga tccttgtaaa gtggatagca cttgtcctga agtcatcaag
      181 gtgtacattt ttaaagctga ccctggagaa gatgacttag gtggaactgt agacattgtg
      241 gagagtgaac ctgaaaatga tcatggagtt gaactacttg atcagaacag cagtattcgt
      301 gttcccaggg aaaagatggt ttatatgact gtcaatgact ctcaacaaga agatgaagat
      361 ttaaatgttg ctgaaattgc tgatgaagtt tatatggaag tgatcgtagg agaggaggat
      421 gctgctgttg cagcagcagc agctgctgtg catgagcagc aaattgatga ggatgaaatg
      481 aaaaccttcg taccaattgc atgggcagca gcttatggta ataattctga tggaattgaa
      541 aaccggaatg gcactgcaag tgccctcttg cacatagatg agtctgctgg ccttggcaga
      601 ctggctaaac agaaaccaaa gaaaaagaga agacctgatt ccaggcagta ccaaacagca
      661 ataattattg gccctgatgg tcatcctttg actgtctatc cttgcatgat ttgtgggaag
      721 aagtttaagt cgaggggttt tttgaaaaga cacatgaaaa accatcctga acaccttgcc
      781 aagaagaagt accactgtac tgactgtgat tacactacca ataagaagat aagtttacat
      841 aaccacctgg agagccacaa gctgaccagc aaggcagaga aggccattga atgtgatgag
      901 tgtgggaagc atttttctca tgcaggggct ttgtttactc acaaaatggt gcataaggaa
      961 aaaggggcca acaaaatgca caagtgtaaa ttctgtgaat atgagacagc tgaacagggg
     1021 ttattgaatc gccacctctt ggcagtccac agcaagaact ttcctcatat ttgtgtggag
     1081 tgtggtaaag gtttccgaca cccgtcggaa ctgagaaagc acatgcgaat ccataccggc
     1141 gagaagccat accaatgcca gtactgtgaa tataggtctg cagactcttc taacttgaaa
     1201 acacatataa aaacaaagca tagtaaagag atgccattca agtgtgacat ttgtcttctg
     1261 actttctcag ataccaaaga agtgcagcaa catactcttg tccaccaaga aagcaaaaca
     1321 catcagtgtt tgcattgcga ccacaagagt tcaaactcaa gtgatttgaa acgacatgta
     1381 atttcagttc atacgaaaga ctatcctcat aagtgtgaga tgtgcgagaa aggctttcac
     1441 aggccttcag aacttaagaa acatgtggct gtccacaaag gtaaaaaaat gcaccaatgt
     1501 agacattgtg actttaagat tgcagaccca tttgttctaa gtcgccatat tctctcagtt
     1561 cacacaaagg atcttccatt taggtgtaag agatgtagaa agggatttag gcaacaaaat
     1621 gagcttaaaa agcatatgaa gacacacagt ggcaggaaag tatatcagtg tgagtactgt
     1681 gagtatagca ctacagatgc ctcaggcttt aaacggcacg ttatttccat tcatacaaaa
     1741 gactatcctc atcggtgtga gtactgcaag aaaggcttcc gaagaccttc agaaaagaac
     1801 cagcacataa tgagacacca taaagaagtt ggtctgccct agtaatgaac tagtccgtaa
     1861 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga gagtccacta
     1921 caggccgggc ttga
//
