LOCUS       sequence-280016-        2507 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0441
FEATURES             Location/Qualifiers
     source          1..2507
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatgaaga tgaatttgaa ttgcagccac aagagccaaa ctcatttttt
       61 gatggaatag gagctgatgc tacacacatg gatggtgatc agattgttgt ggaaatacaa
      121 gaagcagttt ttgtttctaa tattgtggat tctgacataa ctgtgcataa ctttgttcct
      181 gatgacccag actcagttgt aatccaagat gttgttgaag atgttgtcat agaggaggat
      241 gttcagtgct cagatatctt agaagaggca gatgtatctg aaaatgtcat cattcctgag
      301 caagtgctgg actcagatgt aactgaagaa gtttctttac cacactgcac agtcccagat
      361 gatgttttag cttctgacat tacttcaacc tcaatgtcta tgccagaaca tgttttaacg
      421 agtgaatcca tgcatgtgtg tgacattgga catgttgaac atatggtgca tgatagtgta
      481 gtggaagcag aaatcattac tgatcctctg acgagtgaca tagtttcaga agaagtattg
      541 gtagcagact gtgcccctga agcagtcata gatgccagcg ggatctcagt ggaccagcaa
      601 gataatgaca aagccagctg tgaggactac ctaatgattt cgttggatga tgctggcaaa
      661 atagaacatg atggttccac tggagtgacc atcgatgcag aatcagaaat ggatccttgt
      721 aaagtggata gcacttgtcc tgaagtcatc aaggtgtaca tttttaaagc tgaccctgga
      781 gaagatgact taggtggaac tgtagacatt gtggagagtg aacctgaaaa tgatcatgga
      841 gttgaactac ttgatcagaa cagcagtatt cgtgttccca gggaaaagat ggtttatatg
      901 actgtcaatg actctcaaca agaagatgaa gatttaaatg ttgctgaaat tgctgatgaa
      961 gtttatatgg aagtgatcgt aggagaggag gatgctgctg ttgcagcagc agcagctgct
     1021 gtgcatgagc agcaaattga tgaggatgaa atgaaaacct tcgtaccaat tgcatgggca
     1081 gcagcttatg gtaataattc tgatggaatt gaaaaccgga atggcactgc aagtgccctc
     1141 ttgcacatag atgagtctgc tggccttggc agactggcta aacagaaacc aaagaaaaag
     1201 agaagacctg attccaggca gtaccaaaca gcaataatta ttggccctga tggtcatcct
     1261 ttgactgtct atccttgcat gatttgtggg aagaagttta agtcgagggg ttttttgaaa
     1321 agacacatga aaaaccatcc tgaacacctt gccaagaaga agtaccactg tactgactgt
     1381 gattacacta ccaataagaa gataagttta cataaccacc tggagagcca caagctgacc
     1441 agcaaggcag agaaggccat tgaatgtgat gagtgtggga agcatttttc tcatgcaggg
     1501 gctttgttta ctcacaaaat ggtgcataag gaaaaagggg ccaacaaaat gcacaagtgt
     1561 aaattctgtg aatatgagac agctgaacag gggttattga atcgccacct cttggcagtc
     1621 cacagcaaga actttcctca tatttgtgtg gagtgtggta aaggtttccg acacccgtcg
     1681 gaactgagaa agcacatgcg aatccatacc ggcgagaagc cataccaatg ccagtactgt
     1741 gaatataggt ctgcagactc ttctaacttg aaaacacata taaaaacaaa gcatagtaaa
     1801 gagatgccat tcaagtgtga catttgtctt ctgactttct cagataccaa agaagtgcag
     1861 caacatactc ttgtccacca agaaagcaaa acacatcagt gtttgcattg cgaccacaag
     1921 agttcaaact caagtgattt gaaacgacat gtaatttcag ttcatacgaa agactatcct
     1981 cataagtgtg agatgtgcga gaaaggcttt cacaggcctt cagaacttaa gaaacatgtg
     2041 gctgtccaca aaggtaaaaa aatgcaccaa tgtagacatt gtgactttaa gattgcagac
     2101 ccatttgttc taagtcgcca tattctctca gttcacacaa aggatcttcc atttaggtgt
     2161 aagagatgta gaaagggatt taggcaacaa aatgagctta aaaagcatat gaagacacac
     2221 agtggcagga aagtatatca gtgtgagtac tgtgagtata gcactacaga tgcctcaggc
     2281 tttaaacggc acgttatttc cattcataca aaagactatc ctcatcggtg tgagtactgc
     2341 aagaaaggct tccgaagacc ttcagaaaag aaccagcaca taatgagaca ccataaagaa
     2401 gttggtctgc cctagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     2461 tatatatctt gtggaaagga cgacatacct ttctatgcct cactcca
//
