LOCUS       sequence-280028-        3152 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0458
FEATURES             Location/Qualifiers
     source          1..3152
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggtggc ggtggagaag gcggtggcgg cggcggcagc ggcctcggct
       61 gcggcctccg gggggccctc ggcggcgccg agcggggaga acgaggccga gagtcggcag
      121 ggcccggact cggagcgcgg cggcgaggcg gcccggctca acctgttgga cacttgcgcc
      181 gtgtgccacc agaacatcca gagccgggcg cccaagctgc tgccctgcct gcactctttc
      241 tgccagcgct gcctgcccgc gccccagcgc tacctcatgc tgcccgcgcc catgctgggc
      301 tcggccgaga ccccgccacc cgtccctgcc cccggctcgc cggtcagcgg ctcgtcgccg
      361 ttcgccaccc aagttggagt cattcgttgc ccagtttgca gccaagaatg tgcagagaga
      421 cacatcatag ataacttttt tgtgaaggac actactgagg ttcccagcag tacagtagaa
      481 aagtcaaatc aggtatgtac aagctgtgag gacaacgcag aagccaatgg gttttgtgta
      541 gagtgtgttg aatggctctg caagacgtgt atcagagctc atcagagggt aaagttcaca
      601 aaagaccaca ctgtcagaca gaaagaggaa gtatctccag aggcagttgg tgtcaccagc
      661 cagcgaccag tgttttgtcc ttttcataaa aaggagcagc tgaagctgta ctgtgagaca
      721 tgtgacaaac tgacatgtcg agactgtcag ttgttagaac ataaagagca tagataccaa
      781 tttatagaag aagcttttca gaatcagaaa gtgatcatag atacactaat caccaaactg
      841 atggaaaaaa caaaatacat aaaattcaca ggaaatcaga tccaaaacag aattattgaa
      901 gtaaatcaaa atcaaaagca ggtggaacag gatattaaag ttgctatatt tacactgatg
      961 gtagaaataa ataaaaaagg aaaagctcta ctgcatcagt tagagagcct tgcaaaggac
     1021 catcgcatga aacttatgca acaacaacag gaagtggctg gactctctaa acaattggag
     1081 catgtcatgc atttttctaa atgggcagtt tccagtggca gcagtacagc attactttat
     1141 agcaaacgac tgattacata ccggttacgg cacctccttc gtgcaaggtg tgatgcatcc
     1201 ccagtgacca acaacaccat ccaatttcac tgtgatccta gtttctgggc tcaaaatatc
     1261 atcaacttag gttctttagt aatcgaggat aaagagagcc agccacaaat gcctaagcag
     1321 aatcctgtcg tggaacagaa ttcacagcca ccaagtggtt tatcatcaaa ccagttatcc
     1381 aagttcccaa cacagatcag cctagctcaa ttacggctcc agcatatgca gcaacagcaa
     1441 ccgcctccac gtttgataaa ctttcagaat cacagcccca aacccaatgg accagttctt
     1501 cctcctcatc ctcaacaact gagatatcca ccaaaccaga acataccacg acaagcaata
     1561 aagccaaacc ccctacagat ggctttcttg gctcaacaag ccataaaaca gtggcagatc
     1621 agcagtggac agggaacccc atcaactacc aacagcacat cctctactcc ttccagcccc
     1681 acgattacta gtgcagcagg atatgatgga aaggcttttg gttcacctat gatcgatttg
     1741 agctcaccag tgggagggtc ttataatctt ccctctcttc cggatattga ctgttcaagt
     1801 actattatgc tggacaatat tgtgaggaaa gatactaata tagatcatgg ccagccaaga
     1861 ccaccctcaa acagaacggt ccagtcacca aattcatcag tgccatctcc aggccttgca
     1921 ggacctgtta ctatgactag tgtacacccc ccaatacgtt cacctagtgc ctccagcgtt
     1981 ggaagccgag gaagctctgg ctcttccagc aaaccagcag gagctgactc tacacacaaa
     2041 gtcccagtgg tcatgctgga gccaattcga ataaaacaag aaaacagtgg accaccggaa
     2101 aattatgatt tccctgttgt tatagtgaag caagaatcag atgaagaatc taggcctcaa
     2161 aatgccaatt atccaagaag catactcacc tccctgctct taaatagcag tcagagctct
     2221 acttctgagg agactgtgct aagatcagat gcccctgata gtacaggaga tcaacctgga
     2281 cttcaccagg acaattcctc aaatggaaag tctgaatggt tggatccttc ccagaagtca
     2341 cctcttcatg ttggagagac aaggaaagag gatgacccca atgaggactg gtgtgcagtt
     2401 tgtcaaaacg gaggggaact cctctgctgt gaaaagtgcc ccaaagtatt ccatctttct
     2461 tgtcatgtgc ccacattgac aaattttcca agtggagagt ggatttgcac tttctgccga
     2521 gacttatcta aaccagaagt tgaatatgat tgtgatgctc ccagtcacaa ctcagaaaaa
     2581 aagaaaactg aaggccttgt taagttaaca cctatagata aaaggaagtg tgagcgccta
     2641 cttttatttc tttactgcca tgaaatgagc ctggcttttc aagaccctgt tcctctaact
     2701 gtgcctgatt attacaaaat aattaaaaat ccaatggatt tgtcaaccat caagaaaaga
     2761 ctacaagaag attattccat gtactcaaaa cctgaagatt ttgtagctga ttttagattg
     2821 atctttcaaa actgtgctga attcaatgag cctgattcag aagtagccaa tgctggtata
     2881 aaacttgaaa attattttga agaacttcta aagaacctct atccagaaaa aaggtttccc
     2941 aaaccagaat tcaggaatga atcagaagat aataaattta gtgatgattc agatgatgac
     3001 tttgtacagc cccggaagaa acgcctcaaa agcattgaag aacgccagtt gcttaaatag
     3061 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     3121 aaggacgagt gtgttactca ccgagcctca tg
//
