LOCUS       sequence-280037-        1598 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0464
FEATURES             Location/Qualifiers
     source          1..1598
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatttctc agtaaaggta gacatagaga aggaggtgac ctgccccatc
       61 tgcctggagc tcctgacaga acctctgagc ctagattgtg gccacagctt ctgccaagcc
      121 tgcatcactg caaagatcaa ggagtcagtg atcatctcaa gaggggaaag cagctgtcct
      181 gtgtgtcaga ccagattcca gcctgggaac ctccgaccta atcggcatct ggccaacata
      241 gttgagagag tcaaagaggt caagatgagc ccacaggagg ggcagaagag agatgtctgt
      301 gagcaccatg gaaaaaaact ccagatcttc tgtaaggagg atggaaaagt catttgctgg
      361 gtttgtgaac tgtctcagga acaccaaggt caccaaacat tccgcataaa cgaggtggtc
      421 aaggaatgtc aggaaaagct gcaggtagcc ctgcagaggc tgataaagga ggatcaagag
      481 gctgagaagc tggaagatga catcagacaa gagagaaccg cctggaagaa ttatatccag
      541 atcgagagac agaagattct gaaagggttc aatgaaatga gagtcatctt ggacaatgag
      601 gagcagagag agctgcaaaa gctggaggaa ggtgaggtga atgtgctgga taacctggca
      661 gcagctacag accagctggt ccagcagagg caggatgcca gcacgctcat ctcagatctc
      721 cagcggaggt tgaggggatc gtcagtagag atgctgcagg atgtgattga cgtcatgaaa
      781 aggagtgaaa gctggacatt gaagaagcca aaatctgttt ccaagaaact aaagagtgta
      841 ttccgagtac cagatctgag tgggatgctg caagttctta aagagctgac agatgtccag
      901 tactactggg tggacgtgat gctgaatcca ggcagtgcca cttcgaatgt tgctatttct
      961 gtggatcaga gacaagtgaa aactgtacgc acctgcacat ttaagaattc aaatccatgt
     1021 gatttttctg cttttggtgt cttcggctgc caatatttct cttcggggaa atattactgg
     1081 gaagtagatg tgtctggaaa gattgcctgg atcctgggcg tacacagtaa aataagtagt
     1141 ctgaataaaa ggaagagctc tgggtttgct tttgatccaa gtgtaaatta ttcaaaagtt
     1201 tactccagat atagacctca atatggctac tgggttatag gattacagaa tacatgtgaa
     1261 tataatgctt ttgaggactc ctcctcttct gatcccaagg ttttgactct ctttatggct
     1321 gtgcctccct gtcgtattgg ggttttccta gactatgagg caggcattgt ctcatttttc
     1381 aatgtcacaa accacggagc actcatctac aagttctctg gatgtcgctt ttctcgacct
     1441 gcttatccgt atttcaatcc ttggaactgc ctagtcccca tgactgtgtg cccaccgagc
     1501 tcctagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1561 tgtggaaagg acgaaatcgg cgctaccatg caataccg
//
