LOCUS       sequence-280040-        2228 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0466
FEATURES             Location/Qualifiers
     source          1..2228
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggaatgtc caaatcacac agtttctttg gttatcccct gagcatcttc
       61 ttcatcgtgg tcaatgagtt ttgcgaaaga ttttcctact atggaatgcg agcaatcctg
      121 attctgtact tcacaaattt catcagctgg gatgataacc tgtccaccgc catctaccat
      181 acgtttgtgg ctctgtgcta cctgacgcca attctcggag ctcttatcgc cgactcgtgg
      241 ctgggaaagt tcaagaccat tgtgtcgctc tccattgtct acacaattgg acaagcagtc
      301 acctcagtaa gctccattaa tgacctcaca gaccacaacc atgatggcac ccccgacagc
      361 cttcctgtgc acgtggtgct gtccttgatc ggcctggccc tgatagctct cgggactgga
      421 ggaatcaaac cctgtgtgtc tgcgtttggt ggagatcagt ttgaagaggg ccaggagaaa
      481 caaagaaaca gatttttttc catcttttac ttggctatta atgctggaag tttgctttcc
      541 acaatcatca cacccatgct cagagttcaa caatgtggaa ttcacagtaa acaagcttgt
      601 tacccactgg cctttggggt tcctgctgct ctcatggctg tagccctgat tgtgtttgtc
      661 cttggcagtg ggatgtacaa gaagttcaag ccacagggca acatcatggg taaagtggcc
      721 aagtgcatcg gttttgccat caaaaataga tttaggcatc ggagtaaggc atttcccaag
      781 agggagcact ggctggactg ggctaaagag aaatacgatg agcggctcat ctcccaaatt
      841 aagatggtta cgagggtgat gttcctgtat attccactcc caatgttctg ggccttgttt
      901 gaccagcagg gctccaggtg gacactgcag gcaacaacta tgtccgggaa aatcggagct
      961 cttgaaattc agcccgatca gatgcagacc gtgaacgcca tcctgatcgt gatcatggtc
     1021 ccgatcttcg atgctgtgct gtaccctctc attgcaaaat gtggcttcaa tttcacctcc
     1081 ttgaagaaga tggcagttgg catggtcctg gcctccatgg cctttgtggt ggctgccatc
     1141 gtgcaggtgg aaatcgataa aactcttcca gtcttcccca aaggaaacga agtccaaatt
     1201 aaagttttga atataggaaa caataccatg aatatatctc ttcctggaga gatggtgaca
     1261 cttggcccaa tgtctcaaac aaatgcattt atgacttttg atgtaaacaa actgacaagg
     1321 ataaacattt cttctcctgg atcaccagtc actgctgtaa ctgacgactt caagcagggc
     1381 caacgccaca cgcttctagt gtgggccccc aatcactacc aggtggtaaa ggatggtctt
     1441 aaccagaagc cagaaaaagg ggaaaatgga atcagatttg taaatacttt taacgagctc
     1501 atcaccatca caatgagtgg gaaagtttat gcaaacatca gcagctacaa tgccagcaca
     1561 taccagtttt ttccttctgg cataaaaggc ttcacaataa gctcaacaga gattccgcca
     1621 caatgtcaac ctaatttcaa tactttctac cttgaatttg gtagtgctta tacctatata
     1681 gtccaaagga agaatgacag ctgccctgaa gtgaaggtgt ttgaagatat ttcagccaac
     1741 acagttaaca tggctctgca aatcccgcag tattttcttc tcacctgtgg cgaagtggtc
     1801 ttctctgtca cgggattgga attctcatat tctcaggctc cttccaacat gaagtcggtg
     1861 cttcaggcag gatggctgct gaccgtggct gttggcaaca tcattgtgct catcgtggca
     1921 ggggcaggcc agttcagcaa acagtgggcc gagtacattc tatttgccgc gttgcttctg
     1981 gtcgtctgtg taatttttgc catcatggct cggttctata cttacatcaa cccagcggag
     2041 atcgaagctc aatttgatga ggatgaaaag aaaaacagac tggaaaagag taacccatat
     2101 ttcatgtcag gggccaattc acagaaacag atgtagtaat gaactagtcc gtaacttgaa
     2161 agtatttcga tttcttggct ttatatatct tgtggaaagg acgatctgta atgctcaccg
     2221 aactacat
//
